Microbiological characterisation of Burkholderia cepacia isolates from cystic fibrosis patients:: investigation of the exopolysaccharides produced
Microbiological characterisation of Burkholderia cepacia isolates from cystic fibrosis patients:: investigation of the exopolysaccharides produced
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DOI：
10.1111/j.1574-6968.2002.tb11116.x
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发表时间：
2002-03-19
影响因子：
2.1
通讯作者：
Cescutti, P
中科院分区：
文献类型：
--
作者：
Lagatolla, C;Skerlavaj, S;Cescutti, P
从临床标本中直接分离出11株洋葱伯克霍尔德菌：10株来自囊性纤维化患者的痰，1株来自阴道拭子。使用API 20 NE对其进行生化鉴定，并通过基于PCR的测定进行确认。通过特异性PCR扩增获得的基因型表征显示7株属于基因型I，3株属于基因型IIIA，1株属于基因型IV。所有分离株也分型核糖体分型和随机扩增多态性DNA分析。一些特征的菌株进行了检查的能力，产生胞外多糖，与相关的基因型与胞外多糖结构的目的。通过甲基化分析和H-1-NMR光谱分析多糖，以确定结构相似性。结果表明，不同的菌株能够产生化学上不同的多糖。(C)2002年由Elsevier Science B. V.代表欧洲微生物学会联合会出版。
Eleven strains of Burkholderia cepacia were isolated directly from clinical specimens: 10 from sputum of cystic fibrosis patients, and one from a vaginal swab. They were biochemically identified using API20NE and confirmed by a PCR-based assay. The genomovar characterisation obtained by specific PCR amplification revealed seven strains belonging to genomovar I, three belonging to genomovar IIIA and one belonging to genomovar IV. All isolates were also typed by ribotyping and random amplification of polymorphic DNA analysis. Some of the characterised strains were examined for the ability to produce exopolysaccharides, with the aim of correlating the genomovar with the exopolysaccharide structure. The polysaccharides were analysed by means of methylation analysis and H-1-NMR spectroscopy in order to determine structural similarities. It was shown that different strains are capable of producing chemically different polysaccharides. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.