Dipeptidyl peptidase IV (DDP IV) in NASH patients
Dipeptidyl peptidase IV (DDP IV) in NASH patients
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DOI：
10.1016/s1665-2681(19)31905-2
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发表时间：
2007-10-01
影响因子：
3.8
通讯作者：
Tatar, Gonca
中科院分区：
文献类型：
--
作者：
Balaban, Yasemin H.;Korkusuz, Petek;Tatar, Gonca
目的(S)：非酒精性脂肪性肝炎(NASH)是一种病因不明的慢性肝病。胰岛素抵抗、免疫机制和氧化应激是其发病的主要因素。二肽基肽酶IV(DPPIV)或CD26是一种具有内分泌和免疫功能的蛋白质。本研究旨在探讨NASH患者DPPIV的相关变化。方法：检测31例NASH患者和17例健康人血、尿DPPIV活性。对29例患者的肝活检组织进行CD26免疫标记。结果：患者平均年龄46+/-11岁，其中女性14例(45%)。患者组血清DPPIV活性(57.3+/-7.8U/L)明显高于对照组(43.6+/-10.6U/L)(p<；0.0001)，且与组织学分级(p=0.038，r=0.373)和肝细胞病变(p=0.018，r=0.423)相关，而与分期(p=0.286)、分级(p=0.286)和CD26染色(p=0.743)无关。患者组尿DPPIV活性(1.52+/-0.94U/mmolcreatinine)与对照组(1.37+/-0.68U/mmoScinine)相似(p=0.861)。3个肝腺泡区CD26表达水平相同(p=0.076)。CD26的表达强度与组织学分级(p=0.001)和肝性骨病(p=0.003)相关，但与分期和分级无关(p=0.610和0.956)。结论：血清DPPIV活性和CD26在肝脏中的染色强度与NASH和肝骨病的组织病理分级有关。DPPIV可作为一种新的候选基因，在NASH的发病机制中具有多种潜在功能。
Objective(s): Non-alcoholic steatohepatitis (NASH) is a chronic liver disease with unknown etiology. The insulin resistance, immune mechanisms and oxidative stress are the main factors in its pathogenesis. Dipeptidyl peptidase IV (DPPIV) or CD26 is a protein with endocrine and immune functions. This study aimed to elicudate the changes related to DPPIV in NASH patients. Methods: Serum and urinary DPPIV activities were measured in 31 NASH patients and 17 healthy controls. The liver biopsies of 29 patients were immunolabeled for CD26. Results: The mean age of patients were 46 +/- 11 years and 14 (45%) of them were female. The serum DPPIV activity was higher in patients (57.3 +/- 7.8 U/L) than controls (43.6 +/- 10.6 U/L) (p < 0.0001), and correlated with the histopathological grade (p = 0.038, r = 0.373) and hepatosteatosis (p = 0.018, r = 0.423) but not with stage (p = 0.286), class (p = 0.286) or CD26 staining (p = 0.743). The urinary DPPIV activity was similar in patients (1.52 +/- 0.94 U/mmol creatinine) and controls (1.37 +/- 0.68 U/mmol creatinine) (p = 0.861). Three acinar zones of liver had equal CD26 expression (p = 0.076). The intensity of CD26 immunostaining was correlated with histopathological grade (p = 0.001) and hepatosteatosis (p = 0.003) but no correlation with stage or class could be detected (p = 0.610 and 0.956, respectively). In Conclusions: The serum DPPIV activity and the staining intensity of CD26 in liver are correlated with histopathologic grade of NASH and hepatosteatosis. DPPIV can be proposed as a novel candidate with several potential functions in NASH pathogenesis.