Evidence that lymphokine-activated killer cells and natural killer cells are distinct based on an analysis of congenitally immunodeficient mice.
Evidence that lymphokine-activated killer cells and natural killer cells are distinct based on an analysis of congenitally immunodeficient mice.
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基于对先天性免疫缺陷小鼠的分析，证明淋巴因子激活的杀伤细胞和自然杀伤细胞是不同的。
DOI：
10.4049/jimmunol.135.5.2911
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发表时间：
1985
影响因子：
4.4
通讯作者：
S. Rosenberg
中科院分区：
文献类型：
--
作者：
G. Andriole;J. Mulé;C. Hansen;W. M. Linehan;S. Rosenberg
淋巴样细胞在rIL-2中的体外孵育可产生广泛裂解为自体、同基因和同种异体新鲜肿瘤细胞的LAK细胞，但不裂解新鲜的正常细胞。用IL-2体外孵育先天性免疫缺陷小鼠品系，检测NK细胞的存在及其产生LAK细胞的能力。两个免疫缺陷品系(NIH-Beige-Nude和NIH-Beige-Nude-xid)的脾细胞不能产生LAK细胞，但有明显的活性。另一个免疫缺陷小鼠品系(NIH-Beige-xid)的脾细胞产生LAK细胞，但不显示NK细胞活性。在免疫缺陷株中，LAK细胞和NK细胞激活的这种分离表明LAK和NK细胞裂解系统是不同的。
The in vitro incubation of lymphoid cells in RIL 2 results in the generation of LAK cells that are broadly lytic to autologous, syngeneic, and allogeneic fresh tumor cells, but which do not lyse fresh, normal cells. Strains of mice with congenital immunodeficiencies were tested both for the presence of NK cells and for their capacity to generate LAK cells after in vitro incubation with IL 2. Splenocytes obtained from two immunodeficient mouse strains (NIH-Beige-Nude and NIH-Beige-Nude-XID) failed to generate LAK cells, but displayed significant activity. Splenocytes from another immunodeficient mouse strain (NIH-Beige-XID) generated LAK cells but did not display NK cell activity. This dissociation of activation of LAK cells from NK cells among the immunodeficient strains indicates that the LAK and NK cell lytic systems are distinct.