Evidence for adenylate nucleotide transport (ATP-ADP translocation) in vesicles of Frankia sp. strain EAN1pec.
Evidence for adenylate nucleotide transport (ATP-ADP translocation) in vesicles of Frankia sp. strain EAN1pec.
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Frankia sp. 囊泡中腺苷酸核苷酸转运（ATP-ADP 易位）的证据。
DOI：
10.1128/jb.170.7.3053-3057.1988
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发表时间：
1988
影响因子：
3.2
通讯作者：
Ensign,JC
中科院分区：
文献类型：
--
作者：
Tisa,LS;Ensign,JC
苍术糖苷和羧基苍术糖苷部分抑制Frankia菌株EAN1pec分离小泡的固氮酶活性(乙炔还原)。破裂小泡的提取物显示出不受抑制剂影响的固氮酶活性。这些囊泡通过苍术皂苷敏感的机制积累ATP。当小泡被洗涤剂渗透时，这种对ATP摄取的抑制被逆转。过量的ATP和ADP可抑制ATP的摄取，但不能抑制AMP或腺苷的摄取，也可被钙依赖的ATPase抑制剂抑制。钙离子刺激摄取。腺苷三磷酸腺苷的积累伴随着腺苷二磷酸和环磷酸腺苷的释放。以氮气为氮源生长的囊泡和细胞摄取的ATP仅以ATP形式存在于相应的细胞池中。这些数据表明，这种生物体的囊泡中存在一个ATP-ADP转位酶系统。讨论了ATP转运在Frankia菌株EAN1pec与植物根瘤共生中的作用。
Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism. This inhibition of ATP uptake was reversed when vesicles were permeabilized by detergent. Uptake of ATP was inhibited by excess ATP and ADP, but not AMP or adenosine, and by a calcium-dependent ATPase inhibitor. Uptake was stimulated by calcium ions. Accumulation of ATP was accompanied by release of ADP and AMP from the vesicles. The ATP taken up by vesicles and cells grown with N2 as the nitrogen source was found in the corresponding cell pools only as ATP. The data indicate activity of an ATP-ADP translocase system in vesicles of this organism. The role of ATP translocation in the symbiosis between Frankia strain EAN1pec and plant root nodules is discussed.