G1/ELE Functions in the Development of Rice Lemmas in Addition to Determining Identities of Empty Glumes.
G1/ELE Functions in the Development of Rice Lemmas in Addition to Determining Identities of Empty Glumes.
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除了确定空颖的身份外，G1/ELE 在水稻外稃的发育中也发挥作用
DOI：
10.3389/fpls.2016.01006
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发表时间：
2016
影响因子：
5.6
通讯作者：
Shi C
中科院分区：
文献类型：
--
作者：
Liu M;Li H;Su Y;Li W;Shi C
水稻空颖，根据不同的解释也称为不育外稃或基本外稃，在形态和细胞模式上与外稃不同。一致地，控制外稃发育的分子机制与空颖的不同。水稻LEAFY HULL STERILE1（OsLHS1）和DROOPING LEAF（DL）分别调节外稃的细胞模式和维管束数量，而LONG STERILE LEMMA1（G1）/ELONGATED EMPTY GLUME（ELE）和PANICLE PHYTOMER2（PAP2）/OsMADS34决定空颖的身份。尽管已经取得了一些进展，但空颖片的身份仍不清楚，外稃基因和颖片基因之间的遗传相互作用也很少被阐明。在本研究中，鉴定了一个新的 G1/ELE 突变体 g1-6 并分析了其表型。与之前报道的 G1/ELE 突变系类似，g1-6 植物的空颖片转化为外稃状器官。此外，单突变和双突变植物的表型表明，除了之前描述的基因特异性功能外，G1/ELE 和 OsLHS1 在控制空颖片和外稃的维管束数量、细胞体积和细胞层数方面发挥着冗余作用。同时，G1/ELE在osmads1-z花中和OsLHS1在g1-6花中的表达模式表明它们在转录水平上并不相互调节。最后，g1-6植物中空颖片基因OsMADS34/PAP2的下调和外稃基因DL的异位表达进一步证明空颖片是不育外稃。总的来说，我们的研究结果为更好地理解 G1 和 OsLHS1 在花发育和空颖的特性中的功能提供了有价值的信息。
Rice empty glumes, also named sterile lemmas or rudimentary lemmas according to different interpretations, are distinct from lemmas in morphology and cellular pattern. Consistently, the molecular mechanism to control the development of lemmas is different from that of empty glumes. Rice LEAFY HULL STERILE1(OsLHS1) and DROOPING LEAF(DL) regulate the cellular pattern and the number of vascular bundles of lemmas respectively, while LONG STERILE LEMMA1 (G1)/ELONGATED EMPTY GLUME (ELE) and PANICLE PHYTOMER2 (PAP2)/OsMADS34 determine identities of empty glumes. Though some progress has been made, identities of empty glumes remain unclear, and genetic interactions between lemma genes and glume genes have been rarely elucidated. In this research, a new G1/ELE mutant g1–6 was identified and the phenotype was analyzed. Similar to previously reported mutant lines of G1/ELE, empty glumes of g1–6 plants transform into lemma-like organs. Furthermore, Phenotypes of single and double mutant plants suggest that, in addition to their previously described gene-specific functions, G1/ELE and OsLHS1 play redundant roles in controlling vascular bundle number, cell volume, and cell layer number of empty glumes and lemmas. Meanwhile, expression patterns of G1/ELE in osmads1-z flowers and OsLHS1 in g1–6 flowers indicate they do not regulate each other at the level of transcription. Finally, down-regulation of the empty glume gene OsMADS34/PAP2 and ectopic expression of the lemma gene DL, in the g1–6 plants provide further evidence that empty glumes are sterile lemmas. Generally, our findings provided valuable information for better understanding functions of G1 and OsLHS1 in flower development and identities of empty glumes.