Chemical sensing by a novel electrical oscillator: detection and quantitation of polysaccharides in concanavalin A solutions.
Chemical sensing by a novel electrical oscillator: detection and quantitation of polysaccharides in concanavalin A solutions.
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通过新型电振荡器进行化学传感：刀豆球蛋白 A 溶液中多糖的检测和定量。
DOI：
10.1016/0006-291x(86)90321-9
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
T. Omochi
中科院分区：
文献类型：
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作者：
K. Yoshikawa;T. Omochi
测量了在不同浓度的甘露聚糖、淀粉和右旋糖苷的HEPES刀豆蛋白A(ConA)缓冲溶液中，连接到外部电路的两个铂电极上的电振荡。在10−4~1wv%范围内，振荡频率几乎与甘露聚糖浓度的对数成线性关系，而葡聚糖的振荡频率几乎保持不变。对于淀粉，它随着浓度的增加而略有增加。该方法可用于抗原-抗体反应的定量和选择性测定，也可用于检测具有特定结合部位的各种细胞，如癌细胞。
Electrical oscillations across two platinum electrodes connected to an external circuit and immersed in HEPES buffer solutions of concanavalin A (Con A) were measured at various concentrations of mannan, starch and dextran. The frequency of oscillations was found to change almost linearly with the logarithm of the concentration of mannan between 10− 4 and 1 w v%; whereas the frequency remained nearly constant with dextran. With starch it slightly increased as the concentration increased. This method was suggested to be useful for quantitative and selective measurement of antigen-antibody reaction and also for detection of various cells with the specific binding site such as cancer cells.