Generating chimeric zebrafish embryos by transplantation.
Generating chimeric zebrafish embryos by transplantation.
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DOI：
10.3791/1394
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发表时间：
2009-07-17
期刊：
影响因子：
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通讯作者：
Moens, Cecilia
中科院分区：
文献类型：
--
作者：
Kemp, Hilary A;Carmany-Rampey, Amanda;Moens, Cecilia
One of the most powerful tools used to gain insight into complex developmental processes is the analysis of chimeric embryos. A chimera is defined as an organism that contains cells from more than one animal; mosaics are one type of chimera in which cells from more than one genotype are mixed, usually wild-type and mutant. In the zebrafish, chimeras can be readily made by transplantation of cells from a donor embryo into a host embryo at the appropriate embryonic stage. Labeled donor cells are generated by injection of a lineage marker, such as a fluorescent dye, into the one-cell stage embryo. Labeled donor cells are removed from donor embryos and introduced into unlabeled host embryos using an oil-controlled glass pipette mounted on either a compound or dissecting microscope. Donor cells can in some cases be targeted to a specific region or tissue of the developing blastula or gastrula stage host embryo by choosing a transplantation site in the host embryo based on well-established fate maps.
