The mechanical response of talin.
The mechanical response of talin.
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DOI：
10.1038/ncomms11966
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发表时间：
2016-07-07
影响因子：
16.6
通讯作者：
Yan J
中科院分区：
文献类型：
--
作者：
Yao M;Goult BT;Klapholz B;Hu X;Toseland CP;Guo Y;Cong P;Sheetz MP;Yan J
Talin是整合素介导的细胞粘附所必需的一种承载力的细胞质适配器，它将肌动蛋白细胞骨架连接到质膜上基于整合素的细胞-细胞外基质粘附。它的C-末端杆结构域，其中包含13个螺旋束，在细胞粘附和伸展过程中的机械传感中起着重要作用。然而，如何利用的结构稳定性和过渡动力学的13个螺旋束的塔林在不同的塔林依赖性mechanosensing过程仍然知之甚少。在这里，我们报告的所有塔林杆域的力依赖性展开和重折叠动力学。使用实验确定的动力学参数，我们确定了在生理相关的拉动速度和实验测量的延伸波动轨迹下，拉伸塔林的力波动的动态。我们的研究结果表明，力依赖性随机展开和折叠的塔林杆域使塔林一个非常有效的力缓冲器，设置一个生理力的范围只有几个pNs的塔林介导的力传递途径。 Talin是一种机械感应细胞质适配器，将整合素细胞粘附受体连接到肌动蛋白细胞骨架。在这里，作者测量所有塔林杆域的力依赖性折叠和重折叠动力学，提出塔林可以作为力缓冲生理相关条件下。
Talin, a force-bearing cytoplasmic adapter essential for integrin-mediated cell adhesion, links the actin cytoskeleton to integrin-based cell–extracellular matrix adhesions at the plasma membrane. Its C-terminal rod domain, which contains 13 helical bundles, plays important roles in mechanosensing during cell adhesion and spreading. However, how the structural stability and transition kinetics of the 13 helical bundles of talin are utilized in the diverse talin-dependent mechanosensing processes remains poorly understood. Here we report the force-dependent unfolding and refolding kinetics of all talin rod domains. Using experimentally determined kinetics parameters, we determined the dynamics of force fluctuation during stretching of talin under physiologically relevant pulling speeds and experimentally measured extension fluctuation trajectories. Our results reveal that force-dependent stochastic unfolding and refolding of talin rod domains make talin a very effective force buffer that sets a physiological force range of only a few pNs in the talin-mediated force transmission pathway. Talin is a mechanosensing cytoplasmic adaptor that links integrin cell adhesion receptors to the actin cytoskeleton. Here the authors measure the force-dependent folding and refolding kinetics of all talin rod domains to propose that talin can function as a force buffer under physiologically relevant conditions.