Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells
Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells
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DOI：
10.1016/j.febslet.2009.02.031
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发表时间：
2009-03-18
期刊：
影响因子：
3.5
通讯作者：
Nakao, Kazuwa
中科院分区：
文献类型：
--
作者：
Taura, Daisuke;Noguchi, Michio;Nakao, Kazuwa
诱导性多能干细胞（iPS）最近从人成纤维细胞建立。在本研究中，我们研究了四个人iPS细胞系的成脂分化特性，并将其与两个人胚胎干（ES）细胞系的成脂分化特性进行比较。在12天的胚状体形成和另外10天的分化后，在聚-L-鸟氨酸和纤连蛋白包被的培养皿上用成脂分化培养基，人iPS细胞表现出脂质积累和脂肪生成相关分子如C/EBP α、PPAR γ 2、瘦素和aP 2的转录。这些结果表明，人iPS细胞具有与人ES细胞相当的成脂潜力。(C)2009年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-L-ornithine and fibronectin-coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBP alpha, PPAR gamma 2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.