Expression profiles of micro RNA in proliferating and differentiating 32D murine myeloid cells
Expression profiles of micro RNA in proliferating and differentiating 32D murine myeloid cells
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DOI：
10.1002/jcp.20613
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发表时间：
2006-06
影响因子：
5.6
通讯作者：
Bin Shi;M. Prisco;G. Calin;Changfeng Liu;G. Russo;A. Giordano;R. Baserga
中科院分区：
文献类型：
--
作者：
Bin Shi;M. Prisco;G. Calin;Changfeng Liu;G. Russo;A. Giordano;R. Baserga
32D细胞是在白介素3(IL-3)中无限期生长的小鼠髓系细胞。在这些细胞中，I型胰岛素样生长因子(IGF-I)和粒细胞集落刺激因子(G-CSF)诱导向粒细胞分化。32D细胞不表达IGF-I受体的对接蛋白--胰岛素受体底物-1(IRS-1)或IRS-2。IRS-1在这些细胞中的异位表达抑制了分化，细胞变得不依赖IL-3和IGF-1，并可以在小鼠体内形成肿瘤。32D和32D来源的细胞为研究与持续增殖或分化相关的Micro RNA(MiR)的表达谱提供了一个很好的模型。我们在这里介绍了miR微阵列获得的数据，并鉴定了受IGF-1或G-CSF调控的miR，它们与32D小鼠髓系细胞的分化或无限期增殖有关。©2006 Wiley-Liss，Inc.
32D cells are murine myeloid cells that grow indefinitely in Interleukin‐3 (IL‐3). In these cells, the type 1 insulin‐like growth factor (IGF‐I) and granulocytic‐colony stimulating factor (G‐CSF) induce differentiation to granulocytes. 32D cells do not express insulin receptor substrate‐1 (IRS‐1) or IRS‐2, docking proteins of the IGF‐I receptor. Ectopic expression of IRS‐1 in these cells inhibits differentiation, the cells become IL‐3 independent and IGF‐1 dependent and can form tumors in mice. 32D and 32D‐derived cells offer a good model in which to study the expression profiles of Micro Rna (miR) related to sustained proliferation or differentiation. We present here the data obtained with miR micro‐arrays and identify the miR that are regulated by IGF‐1 or G‐CSF and are associated with either differentiation or indefinite cell proliferation of 32D murine myeloid cells. © 2006 Wiley‐Liss, Inc.