Different autonomous myogenic cell populations revealed by ablation of Myf5-expressing cells during mouse embryogenesis
Different autonomous myogenic cell populations revealed by ablation of Myf5-expressing cells during mouse embryogenesis
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DOI：
10.1242/dev.019331
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发表时间：
2008-05-01
期刊：
影响因子：
4.6
通讯作者：
Braun, Thomas
中科院分区：
文献类型：
--
作者：
Gensch, Nicole;Borchardt, Thilo;Braun, Thomas
成肌细胞的发育主要由Myf5和Myod1 （MyoD）两种成肌因子的表达决定，这两种因子在胚胎发生过程中相互补偿。在这里，我们通过条件细胞消融在小鼠中证明Myf5决定了一个独特的肌源性细胞群，其中也包含一些myod1阳性细胞。这个谱系的消失揭示了第二个自主的肌源性谱系的存在，它取代了myf5依赖性的肌源性细胞并表达Myod1。相比之下，消融表达肌原素的细胞几乎消除了所有分化的肌肉细胞，这表明大多数骨骼肌细胞都具有肌生成程序的某些方面。我们得出结论，Myf5和Myod1定义了不同的细胞系，对肌肉前体细胞和分化的肌管有不同的贡献。个体的肌源性细胞系似乎在发育中的胚胎中相互替代。
The development of myogenic cells is mainly determined by expression of two myogenic factors, Myf5 and Myod1 (MyoD), which genetically compensate for each other during embryogenesis. Here, we demonstrate by conditional cell ablation in mice that Myf5 determines a distinct myogenic cell population, which also contains some Myod1-positive cells. Ablation of this lineage uncovers the presence of a second autonomous myogenic lineage, which superseded Myf5-dependent myogenic cells and expressed Myod1. By contrast, ablation of myogenin-expressing cells erased virtually all differentiated muscle cells, indicating that some aspects of the myogenic program are shared by most skeletal muscle cells. We conclude that Myf5 and Myod1 define different cell lineages with distinct contributions to muscle precursor cells and differentiated myotubes. Individual myogenic cell lineages seem to substitute for each other within the developing embryo.