Light sheet microscopy and live imaging of plants
Light sheet microscopy and live imaging of plants
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DOI：
10.1111/jmi.12393
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发表时间：
2016-08-01
影响因子：
2
通讯作者：
Maizel, Alexis
中科院分区：
文献类型：
--
作者：
Berthet, Beatrice;Maizel, Alexis
光片荧光显微镜（LSFM）越来越多地用于研究动物和植物的生物过程。LSFM通过用激光片选择性地照射样品的单个平面来实现光学切片，同时记录与照射平面正交的发射荧光。然后可以生成样本的3D图像，其时间分辨率范围从几秒到几天，并且尺度范围从亚细胞到整个器官。通过设计，LSFM提供快速成像和低光毒性，这是生理条件下实时成像的两个关键标准。尽管LSFM具有潜力，但在植物生物学中仍未得到充分利用。本次审查的目的是突出在植物中的实时成像的挑战，描述在使用LSFM对活的植物样品的关键步骤，最后在提供一个概述LSFM在植物中的应用已公布的例子。
Light sheet fluorescence microscopy (LSFM) is increasingly used to investigate biological processes in animals as well as in plants. LSFM achieves optical sectioning by the selective illumination of a single plane of the sample with a sheet of laser light while simultaneously recording emitted fluorescence orthogonally to the illumination plane. A 3D image of the sample can then be generated with a temporal resolution ranging from seconds to several days, and at scales ranging from subcellular to whole organ. By design, LSFM provides fast imaging, and low phototoxicity, two key criteria for live imaging under physiological conditions. Despite its potential, LSFM remains underutilized in plant biology. This review aims to highlight challenges of live imaging in plants, to describe key steps in using LSFM on live plant samples and finally at providing an overview of published examples of applications of LSFM in plants.