Sequential SPECT and NIR-II Imaging of Tumor and Sentinel Lymph Node Metastasis for Diagnosis and Image-Guided Surgery
Sequential SPECT and NIR-II Imaging of Tumor and Sentinel Lymph Node Metastasis for Diagnosis and Image-Guided Surgery
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肿瘤和前哨淋巴结转移的连续 SPECT 和 NIR-II 成像用于诊断和图像引导手术
DOI：
10.1039/d1bm00088h
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发表时间：
--
影响因子：
6.6
通讯作者：
Yi Y
中科院分区：
文献类型：
--
作者：
Xiaolu Zhang;Meng Zhao;Ling Wen;Manran Wu;Yi Y
有效的癌症治疗很大程度上依赖于准确的成像诊断和成像引导手术，这可以通过在单个纳米平台上组合不同模式的成像探针来实现。本文开发了一种新型放射性标记NIR-II纳米探针（125I-MT NP），用于实现多用途单光子发射计算机断层扫描（SPECT）和第二近红外（NIR-II）荧光双模成像，以对抗乳腺癌。用两亲性三嵌段共聚物（PEO-PPO-PEO）沉淀125I-MT，形成125I-MT NPs。125I-MT纳米粒子具有较高的标记效率（98±2%），其水动力直径为91.3±5.5 nm。体外和体内研究表明，125I-MT NPs发出强烈的NIR-II荧光和SPECT信号，具有良好的生物相容性。通过静脉注射125I-MT NPs后的乳腺肿瘤异种移植小鼠模型，分别在注射后8 h和48 h的SPECT成像和NIR-II成像显示肿瘤组织清晰，提示术前使用125I-MT NPs检测肿瘤和术中可视化解剖区域的可行性。此外，在注射后1小时进行淋巴结显像的SPECT扫描，在注射后4小时进行NIR-II荧光成像。这进一步保证了淋巴结切除术术前和术中淋巴结的准确成像。总体125I-MT NP是一种有前景的、实用的成像探针，用于序列成像和精确癌症治疗。
Efficacious cancer treatment largely relies on accurate imaging diagnosis and imaging-guided surgery, which can be achieved by combining different mode imaging probes on one single nanoplatform. Herein, a novel radiolabeled NIR-II nanoprobe (125I-MT NP) was developed to enable versatile single-photon emission computed tomography (SPECT) and second near-infrared (NIR-II) fluorescence dual-modal imaging against breast cancer. 125I-MT was precipitated with an amphiphilic triblock copolymer (PEO-PPO-PEO) to form 125I-MT NPs. The 125I-MT NPs exhibited high labeling efficiency (98 ± 2%) with a hydrodynamic diameter of 91.3 ± 5.5 nm. In vitro and in vivo studies demonstrated that 125I-MT NPs emitted intensive NIR-II fluorescence and SPECT signals, and possessed good biocompatibility. By using a breast tumor xenograft mouse model after intravenous injection of 125I-MT NPs, the SPECT imaging and NIR-II imaging showed clear images of tumor tissues at 8 h and 48 h postinjection, respectively, suggesting the feasibility of using 125I-MT NPs to detect tumors before surgery and visualize the dissection area during surgery. In addition, the SPECT scan of a lymph node mapping was performed at 1 h postinjection and NIR-II fluorescence imaging was carried out at 4 h postinjection. This further guarantees the accurate imaging of lymph nodes before and during surgery for lymphadenectomy. Overall 125I-MT NP is a promising, practical imaging probe for sequential imaging and precision cancer therapy.