Effects of glycosaminoglycans and glycosphingolipids on cytosolic phospholipases A2 from bovine brain.
Effects of glycosaminoglycans and glycosphingolipids on cytosolic phospholipases A2 from bovine brain.
复制标题
糖胺聚糖和鞘糖脂对牛脑胞质磷脂酶 A2 的影响。
DOI：
10.1042/bj2990091
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Horrocks,LA
中科院分区：
文献类型：
--
作者：
Yang,HC;Farooqui,AA;Horrocks,LA
在牛脑中发现了两种形式的非Ca（2+）依赖性胞浆磷脂酶A2活性（110 kDa和39 kDa）。经SephadexG-75柱层析分离。110 kDa的磷脂酶A2与磷脂酰乙醇胺的活性更高，不受糖胺聚糖的影响，而39 kDa的磷脂酶A2与乙醇胺缩醛的活性更高，并明显受到糖胺聚糖的抑制。硫酸乙酰肝素是最有效的抑制剂，其次是硫酸软骨素，透明质酸和肝素。神经节苷脂，特别是GM 3神经节苷脂，但不是其他鞘糖脂，抑制活性的39 kDa磷脂酶A2的剂量依赖性的方式。110 kDa和39 kDa磷脂酶A2的热失活概况为这些胞质酶之间的差异提供了进一步的证据。糖胺聚糖、神经节苷脂和磷脂酶A2之间的相互作用可能参与膜功能的维持。
Two forms of Ca(2+)-independent cytosolic phospholipase A2 activity (110 kDa and 39 kDa) were found in bovine brain. They were separated by Sephadex G-75 column chromatography. The 110 kDa phospholipase A2 was much more active with phosphatidylethanolamine and was not affected by glycosaminoglycans, whereas the 39 kDa phospholipase A2 was much more active with ethanolamine plasmalogen and was markedly inhibited by glycosaminoglycans. Heparan sulphate was the most potent inhibitor, followed by chondroitin sulphate, hyaluronic acid and heparin. Gangliosides, especially the GM3 ganglioside, but not other glycosphingolipids, inhibited the activity of the 39 kDa phospholipase A2 in a dose-dependent manner. The heat-inactivation profiles of the 110 kDa and 39 kDa phospholipases A2 provide further evidence for the differences between these cytosolic enzymes. Interactions between glycosaminoglycans, gangliosides and phospholipases A2 may be involved in the maintenance of membrane function.