Mixed culture of Kluyveromyces marxianus and Candida krusei for single-cell protein production and organic load removal from whey.

Mixed culture of Kluyveromyces marxianus and Candida krusei for single-cell protein production and organic load removal from whey.
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DOI:
10.1016/j.biortech.2014.04.069
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发表时间:
2014-07
影响因子:
11.4
通讯作者:
J. Yadav;J. Bezawada;C. M. Ajila;S. Yan;R. Tyagi;R. Surampalli
J. Yadav;J. Bezawada;C. M. Ajila;S. Yan;R. Tyagi;R. Surampalli
中科院分区:
工程技术1区
文献类型:
--
作者:
J. Yadav;J. Bezawada;C. M. Ajila;S. Yan;R. Tyagi;R. Surampalli

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研究了马克斯克鲁维酵母(Kluyveromycesmarxianus)和克氏假丝酵母(Candidakrusei)混合培养物在提高COD去除率、减少极端条件(高温40 ° C和低pH 3.5)下分批和连续好氧发酵过程中的污染以及以乳清为底物获得高质量单细胞蛋白(SCP)的潜力。单菌和混合菌的分批发酵结果表明,混合菌的COD去除率提高了8.8%,生物产量提高了19%,生产力提高了33%。水力停留时间为24 h时,COD去除率最高为80.2%,生物量为0.17 g/L/h;水力停留时间为6 h时,生物量为0.38 g/L/h,COD去除率最高为34%。结果表明,耐酸和耐热酵母菌的混合培养是一种在极端条件下生产SCP(动物饲料)并同时去除COD的潜在途径。
The study was conducted to evaluate the potential of mixed culture ofKluyveromyces marxianusandCandida kruseito enhance COD removal efficiency, minimize contamination at extreme conditions (high temperature 40 °C and low pH 3.5) during batch and continuous aerobic fermentation and to obtain improved quality single-cell protein (SCP) using whey as substrate. The batch fermentation of mono-culture and mixed culture result showed that the mixed culture resulted in 8.8% higher COD removal efficacy with 19% higher biomass yield and 33% increased productivity. The maximum COD removal 80.2% (including residual protein) was obtained at 24 h HRT with biomass productivity of 0.17 g/L/h; however, maximum biomass productivity of 0.38 g/L/h and 34% COD removal were obtained at 6 h HRT. The results showed that the mixed culture of acid resistance and thermo-tolerant yeasts was a potential way to produce SCP (animal feed) and simultaneous COD removal under extreme operating conditions.