Comparison of supercritical carbon dioxide and soxhlet extraction of lipids from a brown seaweed, Sargassum hemiphyllum (Turn.) C. Ag.
Comparison of supercritical carbon dioxide and soxhlet extraction of lipids from a brown seaweed, Sargassum hemiphyllum (Turn.) C. Ag.
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DOI：
10.1021/jf980346h
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发表时间：
1998-10-01
影响因子：
6.1
通讯作者：
Ang, PO
中科院分区：
文献类型：
--
作者：
Cheung, PCK;Leung, AYH;Ang, PO
比较了半叶马尾藻（Sargassum hemiphyllum）超临界CO2萃取和氯仿/甲醇索氏提取的脂肪酸组成。对于压力范围（24.1 - 37.9 MPa）和温度区间（40 - 50 ℃）、37.9 MPa/40 ℃和37.9 MPa/50 ℃得到最高的脂质产率，这与索氏方法相当，并且总的和单独的ω-3脂肪酸的浓度最高，其含量显著高于溶剂提取法（P <0.05）。然而，在较高温度但低压水平（24.1MPa/50 ℃）下的SC-CO2提取得到的脂质产率和脂肪酸浓度显著低于（p <0.05）较低温度但相同压力（24.1MPa/40 ℃）下的提取。二十二碳五烯酸和二十二碳六烯酸的浓度在37.9 MPa/40 ℃下显著高于（p <0.05）在37.9 MPa/50 ℃下。随着压力和溶剂密度的增加，总多不饱和脂肪酸的浓度显著增加（p <0.05），总饱和脂肪酸的浓度显著降低（p <0.05），如饱和/不饱和脂肪酸比所示。
The fatty acid profiles of Lipids extracted Sargassum hemiphyllum by supercritical carbon dioxide (SC-CO2) were compared with those obtained by Soxhlet solvent extraction using chloroform/methanol. For the pressure range (24.1-37.9 MPa) and temperature interval (40-50 degrees C), 37.9 MPa/ 40 degrees C and 37.9 MPa/50 degrees C gave the highest lipid yield, which was comparable to that of the Soxhlet method, and the highest concentrations of total and individual omega-3 fatty acids, which were significantly higher (p < 0.05) than the solvent extraction. However, SC-CO2 extraction at higher temperature but low-pressure levels (24.1 MPa/50 degrees C) gave significantly lower (p < 0.05) lipid yield and fatty acid concentration than that of lower temperature but of the same pressure (24.1 MPa/40 degrees C). Concentrations of docosapentaenoic acid and docosahexaenoic acid were significantly higher (p < 0.05) at 37.9 MPa/40 degrees C than at 37.9 MPa/50 degrees C. Concentration of the total polyunsaturated fatty acids increased significantly(p < 0.05) and concentration of total saturated fatty acids decreased significantly(p < 0.05) with increasing pressure and solvent density, as indicated by the saturated/ unsaturated fatty acid ratios.