Hot water extracts of Chlorella vulgaris improve immune function in protein-deficient weanling mice and immune cells
Hot water extracts of Chlorella vulgaris improve immune function in protein-deficient weanling mice and immune cells
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DOI：
10.3109/08923971003604778
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发表时间：
2010-12-01
影响因子：
3.3
通讯作者：
Kim, Hyung-Min
中科院分区：
文献类型：
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作者：
An, Hyo-Jin;Rim, Hong-Kun;Kim, Hyung-Min
本研究的目的是研究普通小球藻（CVE）的热水提取物对通过利用蛋白质-能量营养不良（PEM）饮食而恶化的免疫功能的影响。单细胞藻类C. vulgaris作为生物反应调节剂。PEM与宿主免疫防御能力下降有关。雄性C57 BL/6 J小鼠，最初4周龄，用标准饮食或PEM饮食喂养8天。PEM饲料组小鼠经口给予0.1 g/kg和0.15 g/kg的CVE，持续一周。营养参数，如总蛋白，白蛋白，葡萄糖，和干扰素γ（IFN-γ）的血清中的CVE治疗组相比，非治疗组增加。从脾，浅表，肠系膜淋巴结的单核细胞数量减少，在与PEM饲料喂养的小鼠，但从脾和浅表淋巴结的数量增加的CVE（0.1和0.15 g/kg）的治疗。我们还研究了CVE对人T细胞系、MOLT-4细胞和原代培养的脾细胞中细胞因子产生的影响。与培养基对照相比，CVE处理显著增加了白细胞介素（IL）-2和IL-4的产生，但不影响IFN-γ的产生。结果提示，CVE可能具有改善免疫功能的作用。
The objective of this study was to investigate the effects of hot water extracts of Chlorella vulgaris (CVE) on a deteriorated immune function through utilization of a protein-energy malnutrition (PEM) diet. Unicellular algae, C. vulgaris, were used as biological response modifier. PEM is associated with decreased host immune defense. Male C57BL/6J mice, initially four weeks old, were fed for 8 days with standard diet or a PEM diet. Mice in the PEM diet group were orally administered 0.1 g/kg and 0.15 g/kg of CVE for the following week. Nutritional parameters such as the total protein, albumin, glucose, and interferon gamma (IFN-gamma) were increased in blood serum of the CVE-treated group compared with the non-treated group. The mononuclear cell numbers from spleen, superficial, and mesenteric lymph node were reduced in mice fed with PEM diet, but numbers from the spleen and superficial lymph node were increased by the CVE (0.1 and 0.15 g/kg) treatment. We also investigated the effect of CVE on the production of cytokines in human T-cell line, MOLT-4 cells, and primary cultured splenocytes. The CVE treatment significantly increased the production of both interleukin (IL)-2 and IL-4 compared with the media control, but did not affect the production of IFN-gamma. These results suggest that CVE may be useful in improving the immune function.