A Single-Cell RNA Sequencing Study Reveals Cellular and Molecular Dynamics of the Hippocampal Neurogenic Niche
A Single-Cell RNA Sequencing Study Reveals Cellular and Molecular Dynamics of the Hippocampal Neurogenic Niche
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DOI：
10.1016/j.celrep.2017.11.050
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发表时间：
2017-12-12
期刊：
影响因子：
8.8
通讯作者：
Clevers, Hans
中科院分区：
文献类型：
--
作者：
Artegiani, Benedetta;Lyubimova, Anna;Clevers, Hans
成年鼠齿状回的神经发生发生在一个特殊的微环境中，该微环境在生命中维持神经元的产生。为了充分了解成人神经发生，必须确定神经干细胞（NSC）和祖细胞的发育阶段、它们的分子决定因素以及生态位细胞和分子组成。我们报告了通过分离所有非神经元细胞群对海马生态位进行的单细胞RNA测序研究。我们的分析提供了齿状回细胞的全面描述，并允许识别专属的细胞类型特异性标记。我们定义了NSCs和祖细胞的发育阶段和转录动力学，我们发现，虽然NSCs代表了一个异质的细胞连续体，但祖细胞可以分为不同的亚型。我们确定了少突胶质细胞谱系和转录动力学，并描述了小胶质细胞的转录谱和激活状态。这些综合数据为了解成人神经发生的调控机制提供了宝贵的资源。
Adult neurogenesis in the murine dentate gyrus occurs in a specialized microenvironment that sustains the generation of neurons during life. To fully understand adult neurogenesis, it is essential to determine the neural stem cell (NSC) and progenitor developmental stages, their molecular determinants, and the niche cellular and molecular composition. We report on a single-cell RNA sequencing study of the hippocampal niche, performed by isolating all the non-neuronal cell populations. Our analysis provides a comprehensive description of the dentate gyrus cells, and it allows the identification of exclusive cell-type-specific markers. We define the developmental stages and transcriptional dynamics of NSCs and progenitors, and we find that, while NSCs represent a heterogeneous cellular continuum, progenitors can be grouped into distinct subtypes. We determine the oligodendrocyte lineage and transcriptional dynamics, and we describe the microglia transcriptional profile and activation state. The combined data constitute a valuable resource to understand regulatory mechanisms of adult neurogenesis.