A serpin from the parasitoid wasp Leptopilina boulardi targets the Drosophila phenoloxidase cascade
A serpin from the parasitoid wasp Leptopilina boulardi targets the Drosophila phenoloxidase cascade
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DOI：
10.1016/j.dci.2008.11.013
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发表时间：
2009-01-01
影响因子：
2.9
通讯作者：
Poirie, Marylene
中科院分区：
文献类型：
--
作者：
Colinet, Dominique;Dubuffet, Aurore;Poirie, Marylene
已知昆虫酚氧化酶（PO）级联受丝氨酸蛋白酶抑制剂家族的丝氨酸蛋白酶和丝氨酸蛋白酶抑制剂的严格调控。作为昆虫免疫系统的关键组成部分，它也被怀疑受到几种内寄生蜂的抑制，这些昆虫在其他节肢动物体内发育为宿主。然而，这种抑制的潜在机制在很大程度上是未描述的。在这里，我们的表征编码丝氨酸蛋白酶抑制剂，LbSPNy，高度表达的黄蜂Leptopilino boulardi（ISY型）的毒液中的基因，我们表明，无论是毒液或重组LbSPNy抑制PO级联在果蝇yakuba宿主幼虫的血淋巴。总之，我们的研究结果确定了第一个丝氨酸蛋白酶抑制剂作为毒力因子的寄生蜂，并表明它破坏了激活途径的PO在果蝇宿主。(c)2008爱思唯尔有限公司保留所有权利。
The insect phenoloxiclase (PO) cascade is known to be tightly regulated by serine proteases and serine protease inhibitors of the serpin family. As a key component of the insect immune system, it is also suspected to be inhibited by several endoparasitoid wasps, insects that develop inside other arthropods as hosts. However, the underlying mechanisms of this inhibition are largely undescribed. Here, we the characterization of a gene encoding a serpin, LbSPNy, highly expressed in the venom of the wasp Leptopilino boulardi (ISy type), and we show that either the venom or the recombinant LbSPNy inhibit the PO cascade in the hemolymph of Drosophila yakuba host larva. Altogether, our results identify the first serpin used as a virulence factor by a parasitoid wasp and show that it disrupts the activation pathway the PO in the Drosophila host. (c) 2008 Elsevier Ltd. All rights reserved.