Evidence for phylogeographic structure in Lolium species related to the spread of agriculture in Europe.: A cpDNA study
Evidence for phylogeographic structure in Lolium species related to the spread of agriculture in Europe.: A cpDNA study
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DOI：
10.1007/s001220051461
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发表时间：
2000-07-01
影响因子：
5.4
通讯作者：
Charmet, G
中科院分区：
文献类型：
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作者：
Balfourier, F;Imbert, C;Charmet, G
为了解释两种牧草（多年生黑麦草和多年生黑麦草）自然种群的分布现状，对我国北方地区的两种牧草种群进行了调查。rigidum），我们研究了来自欧洲和中东的51个自然群体的447个植物个体的母系遗传叶绿体DNA（cpDNA）的遗传变异。PCR扩增的cpDNA片段的限制性酶切分析的多态性检测导致在15个单倍型的鉴定。叶绿体多样性的层次分析表明，高水平的种群内的多样性，而这两个物种，我们发现，约40%的总多样性仍然存在于种群之间。利用以前的同工酶数据使我们能够估计花粉与种子流的比例：花粉流似乎是3.5倍，种子流L。perenne和L.僵硬利用群体单倍型频率计算群体间的逐步加权遗传距离。种群的等级聚类清楚地划分了两个种，而两个主要的集群L。多年生植物种群表现出很强的地理结构。提出了不同的方案来解释这两个物种的分布区域。最后，证据证明，这些地理结构有关的农业在欧洲的传播和讨论。
In order to explain the present distribution area of natural populations of two forage grasses species (Lolium perenne and L. rigidum), we studied genetic variation for maternally inherited chloroplast DNA (cpDNA) in 447 individual plants from 51 natural populations sampled throughout Europe and the Middle East. The detection of polymorphism by restriction analysis of PCR-amplified cpDNA fragments resulted in the identification of 15 haplotypes. Hierarchical analysis of chloroplastic diversity showed a high level of within-population diversity while, for both species, we found that about 40% of the total diversity still remains among populations. The use of previous isozymes data enabled us to estimate the pollen to seed flow ratio: pollen flow appears to be 3.5 times greater than seed flow for L. perenne and 2.2 times higher for L. rigidum. A stepwise weighted genetic distance between pairs of populations was calculated using the haplotypes frequencies of populations. A hierarchical clustering of populations clearly divides the two species, while two main clusters of L. perenne populations show a strong geographical structure. Different scenario are proposed for explaining the distribution area of the two species. Finally, evidence attesting that these geographical structures are related to the spread of agriculture in Europe are presented and discussed.