Trehalose promotes the survival of Saccharomyces cerevisiae during lethal ethanol stress, but does not influence growth under sublethal ethanol stress
Trehalose promotes the survival of Saccharomyces cerevisiae during lethal ethanol stress, but does not influence growth under sublethal ethanol stress
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DOI：
10.1111/j.1567-1364.2009.00569.x
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发表时间：
2009-12-01
影响因子：
3.2
通讯作者：
Stanley, Grant A.
中科院分区：
文献类型：
--
作者：
Bandara, Ajith;Fraser, Sarah;Stanley, Grant A.
已知海藻糖可保护细胞免受各种环境攻击;然而，其在酿酒酵母乙醇耐受性中的作用仍存在争议。许多先前的研究报告海藻糖水平与各种菌株的乙醇耐受性之间的相关性，但遗传背景的变化使得难以将海藻糖的影响与其他应激反应因子分开。本研究主要对S.酿酒酵母BY 4742和BY 4742缺失菌株，tsl 1 Delta和nth 1 Delta，在一系列乙醇浓度范围内。结果发现，海藻糖在致死乙醇浓度下对乙醇耐受性起作用，但在亚致死乙醇浓度下不起作用;细胞内海藻糖浓度20-40%的差异对在亚致死乙醇浓度下孵育的细胞没有任何生长优势。据推测，海藻糖效应的乙醇浓度依赖性支持海藻糖保护细胞蛋白免受乙醇损伤作用的机制。
Trehalose is known to protect cells from various environmental assaults; however, its role in the ethanol tolerance of Saccharomyces cerevisiae remains controversial. Many previous studies report correlations between trehalose levels and ethanol tolerance across a variety of strains, yet variations in genetic background make it difficult to separate the impact of trehalose from other stress response factors. In the current study, investigations were conducted on the ethanol tolerance of S. cerevisiae BY4742 and BY4742 deletion strains, tsl1 Delta and nth1 Delta, across a range of ethanol concentrations. It was found that trehalose does play a role in ethanol tolerance at lethal ethanol concentrations, but not at sublethal ethanol concentrations; differences of 20-40% in the intracellular trehalose concentration did not provide any growth advantage for cells incubated in the presence of sublethal ethanol concentrations. It was speculated that the ethanol concentration-dependent nature of the trehalose effect supports a mechanism for trehalose in protecting cellular proteins from the damaging effects of ethanol.