Experimental model of glucocorticoid-induced insulin resistance
Experimental model of glucocorticoid-induced insulin resistance
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DOI：
10.1590/s0102-865020160100000001
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发表时间：
2016-10-01
影响因子：
1.1
通讯作者：
Teixeira, Manoel Araújo
中科院分区：
文献类型：
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作者：
Martínez, Beatriz Bertolaccini;Pereira, Ana Carolina Couto;Teixeira, Manoel Araújo
目的：评价糖皮质激素诱导的胰岛素抵抗实验模型的代谢效应。方法：20只Wistar雄性大鼠随机分为两组，分别腹腔注射地塞米松1mg/Kg/天，连续10 d（D组，n=10）和安慰剂（C组，n=10）。分析的变量是： 从第一天到第十天 - 体重（治疗前和治疗后）；日常消耗的食物和水；第 10 天 - 血糖、胰岛素血症、HOMA-β 和 HOMA-IR。用于实验室分析的血样通过心内穿刺获得。还在第10天取肝碎片用于分析糖原和脂肪。 结果：D组动物与C组相比：体重减轻(g)，(D=226.5+/-24.7 vs C=295.0+/-25.4；p=0.001)；血糖升高（mmol/l）（D=19.5+/-2.1 vs C=14.2+/-3.1；p=0.0001）；胰岛素血症减少（mU/l）（D=0.2+/-0.1 vs C=2.0+/-0.4；p=0.0001）； HOMA-β 降低（D=0.2+/-0.1 对比 C=4.2+/-1.7；p=0.0002）； HOMA-IR 减弱（D=0.2+/-0.1 对比 C=1.3+/-0.4；p=0.0002）。肝脏组织学检查显示D组100%有中度脂肪，C组无中度脂肪。 (p=0.2)。结论：在该实验模型中，用糖皮质激素治疗的动物表现出高血糖、低胰岛素血症和外周胰岛素敏感性降低。
PURPOSE: To evaluate metabolic effects in experimental model of glucocorticoid-induced insulin resistance.METHODS: Twenty Wistar male rats were randomly divided into two groups, which were treated with intraperitoneally injected dexamethasone 1mg/Kg/day for ten days consecutively ( Group D; n=10) and placebo (Group C; n=10). The variables analyzed were: from the first to the 10th day - body weight (before and after treatment); food and water daily consumption; on the 10th day - glycemia, insulinemia, HOMA-beta and HOMA-IR. The blood samples for laboratory analysis were obtained by intracardiac puncture. Also on the 10th day liver fragments were taken for analyzing glycogen and fattty.RESULTS: Group D animals compared to group C had: weight reduction (g), (D=226.5+/-24.7 vs C=295.0+/-25.4; p=0.001); increased glycemia (mmol/l) (D=19.5+/-2.1 vs C=14.2+/-3.1; p=0.0001); diminished insulinemia (mU/l) (D=0.2+/-0.1 vs C=2.0+/-0.4; p=0.0001); reduced HOMA-beta (D=0.2+/-0.1 vs C=4.2+/-1.7; p=0.0002); diminished HOMA-IR (D=0.2+/-0.1 vs C=1.3+/-0.4; p=0.0002). Histological examination of the liver showed that 100% of group D and none of group C had moderate fatty. (p=0.2).CONCLUSION: Animals treated with glucocorticoid, in this experimental model, expressed hyperglycemia, hypoinsulinism and decreased peripheral insulin sensitivity.