Association of Rice Gall Dwarf Virus with Microtubules Is Necessary for Viral Release from Cultured Insect Vector Cells
Association of Rice Gall Dwarf Virus with Microtubules Is Necessary for Viral Release from Cultured Insect Vector Cells
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DOI：
10.1128/jvi.01067-09
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发表时间：
2009-10-15
影响因子：
5.4
通讯作者：
Omura, Toshihiro
中科院分区：
文献类型：
--
作者：
Wei, Taiyun;Uehara-Ichiki, Tamaki;Omura, Toshihiro
通过透射电子、电子断层扫描和共聚焦显微镜发现，感染呼肠孤病毒科成员稻瘿矮病毒的载体昆虫细胞在病毒质附近含有病毒相关的微管。病毒质是病毒复制的假定位点，含有病毒的非结构病毒蛋白Pns7和Pns12以及核心蛋白P5。微管解聚药物抑制病毒颗粒与微管的结合，阻止病毒从细胞中释放，但不显著影响病毒增殖。因此，微管似乎介导了病毒在受感染载体细胞内的转运和病毒的释放。
Vector insect cells infected with Rice gall dwarf virus, a member of the family Reoviridae, contained the virus-associated microtubules adjacent to the viroplasms, as revealed by transmission electron, electron tomographic, and confocal microscopy. The viroplasms, putative sites of viral replication, contained the nonstructural viral proteins Pns7 and Pns12, as well as core protein P5, of the virus. Microtubule-depolymerizing drugs suppressed the association of viral particles with microtubules and prevented the release of viruses from cells without significantly affecting viral multiplication. Thus, microtubules appear to mediate viral transport within and release of viruses from infected vector cells.