Vascular smooth muscle-derived relaxing factor (MDRF) and its close similarity to nitric oxide.
Vascular smooth muscle-derived relaxing factor (MDRF) and its close similarity to nitric oxide.
复制标题
血管平滑肌源性松弛因子（MDRF）及其与一氧化氮的密切相似性。
DOI：
10.1016/0006-291x(90)91243-l
复制
发表时间：
1990
影响因子：
3.1
通讯作者：
Ignarro,LJ
中科院分区：
文献类型：
--
作者：
Wood,KS;Buga,GM;Byrns,RE;Ignarro,LJ
这项研究的主要发现是血管平滑肌产生一种不稳定的松弛因子，其药理和化学性质与真正的一氧化氮相似。 MDRF 是通过生物测定评估的去内皮牛肺动脉灌注产生的。此外，在 37°C 下孵育 24 小时以降低内源性 L-精氨酸水平的内皮剥脱动脉环会响应 L-精氨酸而松弛，但不会响应 D-精氨酸。刚安装的、内皮剥脱的动脉环不会被 L-精氨酸松弛，但会响应二肽 L-精氨酰-L-丙氨酸而松弛。松弛反应伴随着平滑肌环鸟苷酸和亚硝酸盐水平的增加，并被氧合血红蛋白、亚甲蓝和 NG-硝基-L-精氨酸抑制。 NG-硝基-L-精氨酸也引起内皮依赖性收缩反应。因此，血管平滑肌可以产生具有一氧化氮性质的松弛因子。
The principal finding in this study is that vascular smooth muscle generates a labile relaxing factor that possesses pharmacological and chemical properties that are similar to those of authentic nitric oxide. MDRF was generated by perfusion of endothelium-denuded bovine pulmonary artery as assessed by bioassay. In addition, endothelium-denuded arterial rings that were incubated at 37°C for 24 hr to lower endogenous L-arginine levels relaxed in response to L-arginine but not D-arginine. Freshly mounted, endothelium-denuded arterial rings were not relaxed by L-arginine but did relax in response to the dipeptide L-arginyl-L-alanine. Relaxant responses were accompanied by increases in smooth muscle levels of cyclic GMP and nitrite, and were inhibited by oxyhemoglobin, methylene blue, and NG-nitro-L-arginine. NG-Nitro-L-arginine also caused endothelium-independent contractile responses. Thus, a relaxing factor with the properties of nitric oxide can be generated from vascular smooth muscle.