PRESENT ASPECTS OF IMMUNODIAGNOSIS OF SCHISTOSOMIASIS
PRESENT ASPECTS OF IMMUNODIAGNOSIS OF SCHISTOSOMIASIS
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DOI：
10.1590/s0074-02761992000800004
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发表时间：
1992-01-01
影响因子：
2.8
通讯作者：
BERGQUIST, NR
中科院分区：
文献类型：
--
作者：
BERGQUIST, NR
由于分子生物学的进步，血吸虫病的免疫诊断技术得到了促进和改进，包括基于检测血清中循环和/或尿液中排泄的抗原的化验，现已进入多中心试验阶段。有必要补充寄生虫学技术，因为一些国家方案在建立疾病控制方面越来越成功，而传统的方法往往不能发现低强度感染。一些地区的流行病学调查小组已初步开始使用血清学，其经验表明，在预期流行率较低的根除或控制地区，抗体检测就足够了，但在最近控制的地区，需要检测循环抗原，以评估感染或再感染的发生率，在推荐试剂和程序用于国家控制方案的日常使用之前，必须用来自地理上确定的区域的临床上充分表征的患者的血清来标准化测定，因此强调了对参考血清库的需要。由国家公共卫生实验室使用标准化测试系统进行血清学测试，将能够在不同地区之间进行有效的比较，为国家卫生政策的决策提供支持。
Facilitated and improved by advances in molecular biology, techniques for the immunodiagnosis of schistosomiasis, including assays based on the detection of antigens circulating in the serum and/or excreted in the urine, have now reached the stage of multi-centre trials. There is a need to complement parasitological techniques as some national programmes are becoming increasingly successful in establishing control of the disease and the classical approach frequently fails to reveal low-intensity infection. Epidemiological survey teams in some areas have tentatively started to use serology and their experience indicates that antibody detection suffices in eradicated or controlled areas with low expected prevalence but that detection of circulating antigens is needed for assessment of the incidence of infection or reinfection in areas recently brought under control.Before reagents and procedures can be recommended for routine use of national control programmes, the assays must be standardized with sera from clinically well-characterized patients in geographically defined regions, hence emphasizing the need for a reference serum bank. Implementation of serological testing, carried out by national public health laboratories using standardized testing systems, would permit valid comparisons between different areas providing support for decisions regarding national health policies.