Reagentless chemiluminescence flow sensor for the determination of riboflavin in pharmaceutical preparations and human urine.
Reagentless chemiluminescence flow sensor for the determination of riboflavin in pharmaceutical preparations and human urine.
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DOI：
10.1039/b102679h
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Z. Song;L. Wang
中科院分区：
文献类型：
--
作者：
Z. Song;L. Wang
利用化学发光检测技术，将核黄素固定在荧光探针上，建立了一种测定核黄素的连续流动化学发光传感器。结果发现，核黄素的存在下，鲁米诺和高碘酸盐之间的氧化的化学发光强度可以增强。核黄素浓度在0.04 ~ 200 ng·ml ~（-1）范围内与荧光强度呈线性关系，检出限为0.02 ng·ml ~（-1）（3s）。考虑到有效的反应离子，鲁米诺和IO 4-被固定在阴离子交换树脂上。该系统以水为洗脱剂，能产生明显的化学发光信号，大大提高了核黄素测定的选择性和灵敏度，具有较高的信噪比。在0.5min内完成了样品的分析，相对标准偏差小于3.0%。该流量传感器已成功地应用于药物制剂和人尿样中核黄素的测定。
A novel continuous-flow sensor based on chemiluminescence (CL) detection was developed for the determination of riboflavin at pg ml(-1) levels by the immobilization of the reagents. It was found that the CL intensity from the oxidation between luminol and periodate could be enhanced in the presence of riboflavin. The increase of CL emission was correlated with the riboflavin concentration in the range from 0.04 to 200 ng ml(-1), and the detection limit was 0.02 ng ml(-1) (3s). Considering the effective reaction ions, luminol and IO4- was immobilized on anion-exchange resin. The system could produce an evident CL signal by water as eluant and it was also shown that the flow sensor could greatly improve the selectivity and sensitivity for determination of riboflavin with a high signal-to-noise ratio. A complete analysis, including sampling and washing, could be performed in 0.5 min with a relative standard deviation of less than 3.0%. The flow sensor was applied successfully to the determination of riboflavin in pharmaceutical preparations and human urine samples.