Abnormalities of synapses and neurons in the hippocampus of neuropsin-deficient mice
Abnormalities of synapses and neurons in the hippocampus of neuropsin-deficient mice
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DOI：
10.1006/mcne.2000.0945
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发表时间：
2001-03-01
影响因子：
3.5
通讯作者：
Shiosaka, S
中科院分区：
文献类型：
--
作者：
Hirata, A;Yoshida, S;Shiosaka, S
在本研究中，我们生产了neuropsin（一种细胞外基质丝氨酸蛋白酶）的空突变小鼠，以研究这种蛋白质特别是在海马中的神经功能。Golgi-Cox浸渍和NissI染色显示突变小鼠与野生型小鼠相比细胞索马体的形态学改变。然而，Golgi-Cox浸渍显示树突状分支和棘密度没有明显变化。定量电子显微镜分析显示，不对称突触的数量显着减少，在辐射层，主要终端领域的谢弗-侧枝，而自由终扣仍然持有突触囊泡，但没有突触专业化的数量增加，在相同的显微镜领域。在突变体中还观察到小清蛋白免疫反应细胞（称为快速尖峰细胞）数量增加。这些结果强烈表明，neuropsin参与了一组CA 1突触的连接，因此在海马网络。
In the present study,we produced null-mutant mice of neuropsin, an extracellular matrix serine protease, to examine the neural functions of this protein particularly in the hippocampus. Golgi-Cox impregnation and NissI-staining revealed morphological change of cell soma in the mutant mice compared to wild-type mice. However, Golgi-Cox impregnation revealed no apparent change in the dendritic arborization and spine density. Quantitative electronmicroscopic analysis revealed that number of asymmetrical synapses were significantly decreased in the stratum radiatum, the major terminal field of Schaffer-collaterals, whereas free boutons still holding synaptic vesicles but with no synaptic specialization were increased in number in the same microscopic fields. An increased number of parvalbumin-immunoreactive cells (known as fast spiking cells) in mutant was also observed. These results strongly suggest that neuropsin is involved in connectivity of a group of CA1 synapses and consequently in the hippocampal networking.