Magnetic molecularly imprinted polymer for the detection of rhaponticin in Chinese patent medicines
Magnetic molecularly imprinted polymer for the detection of rhaponticin in Chinese patent medicines
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磁性分子印迹聚合物检测中成药中土大黄素
DOI：
10.1016/j.chroma.2012.06.071
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发表时间：
2012-08-24
影响因子：
4.1
通讯作者：
Shi, Yan-Ping
中科院分区：
文献类型：
--
作者：
Chen, Fang-Fang;Xie, Xiao-Yu;Shi, Yan-Ping
合成了磁性分子印迹聚合物（MMIPs），用于中成药中土大黄苷的选择性提取和净化。以Fe 3 O 4为磁性组分，土大黄素为模板分子，丙烯酰胺（AM）为功能单体，苯乙烯（St）为共聚单体，乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，二甲基亚砜为致孔剂，采用悬浮聚合法制备了MMIPs。采用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）和振动样品磁强计（VSM）对MMIPs进行了表征。采用高效液相色谱法（HPLC）对目标物进行分析。通过分批再结合实验进一步评价聚合物。从推导的Freundlich等温线（FI）方程，它们的结合容量和结合强度进行了测定。结构相似的化合物白藜芦醇和参考化合物奇烯醇用于研究MMIPs的选择性识别能力。MMIPs被用来作为选择性的预浓缩从CPM样品中的土大黄苷的吸附剂。4种中成药样品中土大黄苷的含量分别为11.84、3.35、4.47和7.57 μ g g（-1），加标回收率为77.82%~ 91.00%。所制备的MMIPs可用于中成药样品中土大黄苷的选择性预富集和测定。(C)2012爱思唯尔有限公司版权所有。
The magnetic molecularly imprinted polymers (MMIPs) had been synthesized for the selective extraction and clean-up of rhaponticin in Chinese patent medicines (CPM). The MMIPs were prepared via suspension polymerization, using Fe3O4 as magnetically component, rhaponticin as template molecule, acrylamide (AM) as functional monomers, styrene (St) as copolymer monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linker in dimethyl sulfoxide porogen. The obtained MMIPs were characterized using scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). High performance liquid chromatography (HPLC) was used for analysis the target analytes. The polymers were evaluated further by batch rebinding experiments. From the derived Freundlich isotherm (FI) equation their binding capacity and binding strength were determined. Structurally similar compound resveratrol and a reference compound kirenol were used for investing the selective recognition capability of MMIPs. The MMIPs were used as sorbents for selective pre-concentration of rhaponticin from CPM samples. The contents of rhaponticin for the four CPM samples were found to be 11.84, 3.35, 4.47, and 7.57 mu g g(-1), respectively, and the recoveries of spiked samples ranged from 77.82% to 91.00%. The prepared MMIPs could be applied to selectively pre-concentrate and determine rhaponticin in CPM samples. (C) 2012 Elsevier B.V. All rights reserved.