MECHANISM OF RESISTANCE TO SULFITE IN SACCHAROMYCES-CEREVISIAE
MECHANISM OF RESISTANCE TO SULFITE IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1007/bf00326407
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发表时间：
1992-12-01
期刊：
影响因子：
2.5
通讯作者：
POLSINELLI, M
中科院分区：
文献类型：
--
作者：
CASALONE, E;COLELLA, CM;POLSINELLI, M
与其亲本菌株相比，七种酿酒酵母亚硫酸盐抗性独立突变体中由亚硫酸盐引起的生长抑制和细胞杀伤减少。遗传分析表明，在七个突变体中，抗性是作为单基因显性突变遗传的，并且所有分析的突变都是等位基因，从而确定了酿酒酵母中负责亚硫酸盐抗性的主要基因。对其中两个突变体 MBS20-9 和 MBS30 进行了进一步表征。 S-35-亚硫酸盐吸收实验表明，两种抗性菌株积累亚硫酸盐的能力显着降低。就甘油醛-3-磷酸脱氢酶对亚硫酸盐的敏感性而言，抗性菌株和敏感菌株之间没有差异。或对细胞内谷胱甘肽含量进行揭示。相反，无论存在还是不存在亚硫酸盐，抗性突变体的细胞外乙醛浓度都较高。
Growth inhibition and cell killing caused by sulphite were reduced in seven Saccharomyces cerevisiae sulphite-resistant independent mutants, compared to their parental strains. Genetic analysis showed that in the seven mutants resistance was inherited as a single-gene dominant mutation and that all the analyzed mutations were allelic, thus identifying a major gene responsible for sulphite resistance in S. cereviviae. Two of the mutants, MBS20-9 and MBS30, were further characterized. S-35-sulphite uptake experiments showed that the ability to accumulate sulphite was markedly reduced in the two resistant strains. No difference between resistant and sensitive strains with respect to glyceraldehyde-3-phosphate dehydrogenase sensitivity to sulphite. or to intracellular glutathione content, were revealed. In contrast, the extracellular acetaldehyde concentration was higher in the resistant mutants, both in the presence and in the absence of sulphite.