New horizons in reproductive biology: a special issue.
New horizons in reproductive biology: a special issue.
复制标题
生殖生物学的新视野：特刊。
DOI：
10.1093/biolre/ioz138
复制
发表时间：
2019
影响因子：
3.6
通讯作者：
Clarke,Hugh
中科院分区：
文献类型：
--
作者：
Yan,Wei;Clarke,Hugh
桑格测序在称霸30年后，最终被深度、高通量、平行的脱氧核糖核酸（DNA）测序技术所取代，也被称为下一代测序。下一代测序技术已使DNA和核糖核酸（RNA）序列及其化学修饰的全基因组检测变得高效和经济。因此，在鉴定导致许多疾病的基因突变方面取得了重大进展。此外，在过去的十年中，在转录，转录后，翻译和翻译后水平的基因调控的新见解已经获得。作为深度测序技术的进一步发展，单细胞RNA深度测序结合了微流体技术和细胞转录组学分析，代表了最新的技术进步之一。通过对单个细胞的转录组学分析，发现了新的细胞类型或亚型，并开始揭示发育和特定生理过程中基因表达的复杂时空调控。自2012年首次出现以来，基于CRISPR/Cas的基因组编辑已迅速成为最广泛使用的基因组高效精确操作技术。因此，通过动物模型确定数十万个基因的生理作用的速度大大加快。随着技术的不断进步，这项技术不仅成为几乎所有实验室研究的标准工具，而且有望为许多遗传性疾病找到治疗方法。得益于这些新技术，生殖生物学领域在过去的2010年中也取得了重大进展。为了突出我们领域的新视野，我们正在出版一个特刊。我们的一些最好的研究人员接受了我们的邀请，并就重大进展的主题提供了全面的评论，包括基于人工精子的基因组编辑（李劲松），精子体外分化（Katsuhiko Hayashi），生殖道的性别分化（Humphrey Yao）、母胎相互作用（Peter J.汉森）、精子发生（Jeremy Wang）、母合子转换（Heng-Yu Fan）、女性不育（Svetlana A. Yatsenko和Aleksandar Rajkovic）、环境暴露对女性生殖的表观遗传影响（Jodi Flaws）、生精细胞的单细胞RNA测序（Brian Hermann）、卵巢癌（大卫P. Cook和Barbara C. Vanderhyden），以及稀有和濒危物种的繁殖（Pierre Comizzoli）。虽然我们无法在这期特刊中涵盖所有领域，但我们希望您能发现这些未来的观点代表了我们快速发展的领域，这是前所未有的令人兴奋和鼓舞人心。阅读愉快！
After 30 years of dominance, Sanger sequencing was finally replaced by deep, high-throughput, in-parallel deoxyribonucleic acid (DNA) sequencing technologies, also called next-generation sequencing. Next-generation sequencing has made the genome-wide detection of DNA and ribonucleic acid (RNA) sequences and their chemical modifications efficiently and economically. Consequently, significant progress has been made in the identification of gene mutations responsible for many diseases. Moreover, new insights into gene regulation at transcriptional, post-transcriptional, translational, and post-translational levels have been gained over the past decade. As a further development of deep-sequencing technologies, single-cell RNA deep sequencing, which combines microfluidics with singlecell transcriptomic analyses, represents one of the latest technological advances. By transcriptomic profiling of single cells, new cell types or subtypes have been discovered, and the complicated spatiotemporal regulation of gene expression during development and specific physiological processes has begun to be uncovered. Since its initial appearance in 2012, CRISPR/Cas-based genome editing has quickly become the most widely used technology for efficient and precise manipulation of the genome. As a result, the speed at which the physiological roles of hundreds of thousands of genes are defined through animal models has been drastically accelerated. With continuous improvement, this technology not only has become a standard tool for almost all laboratory studies, but also bears the hope for finding cures for many genetic diseases.Benefiting from these new technologies, the field of reproductive biology has also witnessed a significant progress over the past∼ 10 years. To highlight new horizons in our field, we are publishing a special issue. A number of our best investigators have accepted our invitation and contributed comprehensive reviews on topics of significant progress, including artificial spermatid-based genome editing (Jinsong Li), in vitro differentiation of sperm (Katsuhiko Hayashi), sexual differentiation of the reproductive tracts (Humphrey Yao), maternal-fetal interactions (Peter J. Hansen), spermatogenesis (Jeremy Wang), maternal-to-zygotic transition (Heng-Yu Fan), female infertility (Svetlana A. Yatsenko and Aleksandar Rajkovic), epigenetic effects of environmental exposures on female reproduction (Jodi Flaws), single-cell RNA-seq of spermatogenic cells (Brian Hermann), ovarian cancer (David P. Cook and Barbara C. Vanderhyden), and reproduction of rare and endangered species (Pierre Comizzoli). Although we could not cover every area in this special issue, we hope that you find these views of the future representative of our fast-developing field, which has never been this exciting and inspiring. Happy reading!