Phylogenetic and functional diversity of propionate-oxidizing bacteria in an anaerobic digester sludge
Phylogenetic and functional diversity of propionate-oxidizing bacteria in an anaerobic digester sludge
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DOI：
10.1007/s00253-007-0842-y
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发表时间：
2007-06-01
影响因子：
5
通讯作者：
Okabe, Satoshi
中科院分区：
文献类型：
--
作者：
Ariesyady, Herto Dwi;Ito, Tsukasa;Okabe, Satoshi
采用显微放射自显影技术结合荧光原位杂交技术（MAR-FISH）研究了厌氧消化池中共生丙酸盐氧化菌（POB）的系统发育和功能多样性。厌氧消化池污泥中的互养POB社区由至少四个系统发育组：互养，未培养的短杆史密斯（史密斯属SR），未培养的长杆史密斯（史密斯属LR），和一个身份不明的组。这些POB基团的活性依赖于丙酸浓度。未培养的Smithella sp. SR在所有测试的丙酸盐浓度（0.515 mM）下占总活性POB的52-62%。相比之下，未培养的史密斯氏菌LR仅在较低的丙酸盐浓度下才有活性，并在0.5 mM丙酸盐下成为占主导地位的活性POB。共养菌-占总活性POB的16- 31%，高于2.5mM丙酸盐，而活性共养菌群变低（约100%）。6%）。厌氧消化器的操作在一个填充和绘制模式，导致丙酸浓度的周期性变化范围从0到10毫米。这些生物遗传和功能多样，在一定程度上功能冗余，活跃的POB社区动态响应丙酸浓度的周期性变化。
The phylogenetic and functional diversity of syntrophic prop ionate-oxidizing bacteria (POB) present in an anaerobic digester was investigated by microautoradiography combined with fluorescent in situ hybridization (MAR-FISH) that can directly link 16S rRNA phylogeny with in situ metabolic function. The syntrophic POB community in the anaerobic digester sludge consisted of at least four phylogenetic groups: Syntrophobacter, uncultured short rod Smithella (Smithella sp. SR), uncultured long rod Smithella (Smithella sp. LR), and an unidentified group. The activities of these POB groups were dependent on the propionate concentrations. The uncultured Smithella sp. SR accounted for 52-62% of the total active POB under all the propionate concentrations tested (0.515 mM). In contrast, uncultured Smithella sp. LR was active only at lower propionate concentrations and became a dominant active POB at 0.5 mM of propionate. Syntrophobactet- accounted for 16-3 1 % of the total active POB above 2.5 mM propionate, whereas the active Syntrophobacter population became low (ca. 6%) at 0.5 mM of propionate. The anaerobic digester was operated in a fill and draw mode, resulting in periodical changes in propionate concentration ranging from 0 to 10 mM. These phylogenetically and functionally diverse, to some extent functionally redundant, active POB communities were dynamically responding to the periodical changes in propionate concentration.