POSSIBLE FUNCTION OF COATED VESICLES IN MELANOGENESIS OF REGENERATING FOWL FEATHER
POSSIBLE FUNCTION OF COATED VESICLES IN MELANOGENESIS OF REGENERATING FOWL FEATHER
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DOI：
10.1083/jcb.48.1.41
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发表时间：
1971-01-01
影响因子：
7.8
通讯作者：
BRUMBAUG.JA
中科院分区：
文献类型：
--
作者：
MAUL, GG;BRUMBAUG.JA
利用二羟基苯丙氨酸（DOPA）对再生禽类羽毛黑色素细胞中的酪氨酸酶活性进行了组织化学论证，研究了酪氨酸酶是如何与黑素体前体相关的。DOPA的反应产物定位于与高尔基体某些dictysomal凹侧相关的吻合膜小管和大部分与dictysomal相连的包被囊泡中。早期黑素体前未见反应产物。在黑素体前，多巴的反应产物首先出现在直径约400a的囊泡中，这些囊泡被具有周期性特征的基质包围。这些观察结果似乎允许这样的推测，即被包被的囊泡在酪氨酸酶的运输中起作用，并且黑素体前体的形成过程并不一定依赖于先前假设的高尔基体。
How tyrosinase becomes associated with the premelanosomes was investigated by histochemical demonstration of tyrosinase activity by the use of dihydroxyphenylalanine (DOPA) in melanocytes of regenerating fowl feathers. The reaction product of DOPA was localized in the anastomosing membrane tubules associated with the concave side of some dictyosomes of the Golgi apparatus and in coated vesicles most of which were in connection with the dictyosomes. No reaction product was found in early premelanosomes. In premelanosomes, the reaction product of DOPA appears first in vesicles approximately 400 A in diameter which are surrounded by a matrix with a characteristic periodicity. These observations seem to allow the speculation that the coated vesicles function in the transport of tyrosinase, and that the premelanosomes are formed in a process which is not necessarily dependent on the Golgi apparatus as was assumed earlier.