Improvement of Skin Quality after Fat Grafting: Clinical Observation and an Animal Study
Improvement of Skin Quality after Fat Grafting: Clinical Observation and an Animal Study
复制标题
DOI：
10.1097/prs.0b013e3181b17b8f
复制
发表时间：
2009-09-01
影响因子：
3.6
通讯作者：
Damour, Odile
中科院分区：
文献类型：
--
作者：
Mojallal, Ali;Lequeux, Charlotte;Damour, Odile
背景：体积和轮廓缺陷的恢复是整形外科中的一个挑战。自体脂肪组织移植在该领域越来越受欢迎。本研究的目的是探讨动物模型移植脂肪组织后皮肤的组织学改变。方法：实验采用30只裸鼠，分为3组。所有 30 只小鼠的一侧都接受了人体脂肪组织。另一侧，10只小鼠什么也不接受（阴性对照组），10只小鼠接受细胞增殖培养基，其余10只小鼠仅接受皮下隧道疗法。八周后，对皮肤和皮下组织进行活组织检查，并通过苏木精-phloxin-藏红花染色对标本进行分析。测量真皮厚度。为了区分人类和小鼠胶原纤维，使用了人类和小鼠 I 型胶原蛋白抗体。使用 III、V 和 VI 型胶原抗体通过免疫组织化学（免疫染色）研究其他类型的胶原蛋白。结果：在所有动物中均发现脂肪组织。宏观上，脂肪组织呈现正常状态，具有丰富的外周新生血管。组织学检查显示注射的人体脂肪组织周围有丰富的细胞外基质。这是由于移植的人体脂肪组织刺激小鼠成纤维细胞，导致小鼠来源的 I 型胶原纤维增加。脂肪移植后真皮厚度明显增加。这并不是由于炎症反应，因为在我们的对照组中没有检测到任何变化。结论：这项研究表明，脂肪组织移植会刺激受体部位胶原纤维的新合成，并使真皮更厚。然而，长期影响仍不确定，需要进一步研究。 （塑料重建外科 124：765，2009 年。）
Background: Restoration of volume and contour defects is a challenge in plastic surgery. Autologous fat tissue transfer is gaining increasing popularity in this field. The aim of this study was to investigate the histologic modifications of the skin after fat tissue grafting on an animal model.Methods: Thirty nude mice, divided into three groups, were used in the experiment. All 30 mice received human fat tissue on one side. On the opposite side, 10 mice received nothing (negative control group), 10 mice received cell proliferation medium, and the remaining 10 mice received only subcutaneous tunneling. Eight weeks later, biopsies of the skin and subcutaneous tissue were performed and specimens were analyzed by hematoxylin-phloxin-saffron staining. Dermis thickness was measured. To differentiate human from murine collagen fibers, human and murine collagen type I antibodies were used. The other types of collagen were investigated by immunohistochemistry (immunostaining) using collagen type III, V, and VI antibodies.Results: Fat tissue was found in all animals. Macroscopically, fat tissue presented normal aspects, with abundant peripheral neovascularization. Histologic examination showed abundant extracellular matrix around the injected human fat tissue. This was attributable to increased type I collagen fibers of murine origin as a result of the murine fibroblast stimulation by the grafted human fat tissue. Dermal thickness after fat grafting was significantly greater. This was not attributable to inflammatory reactions, because no modification was detected in our control groups.Conclusions: This study shows that fat tissue grafting stimulates a neosynthesis of collagen fibers at the recipient site and makes the dermis thicker. However, the long-term effects remain undetermined and need further investigation. (Plast. Reconstr. Surg. 124: 765, 2009.)