CHLORAMPHENICOL-ERYTHROMYCIN RESISTANCE MUTATIONS IN A 23S RIBOSOMAL-RNA GENE OF ESCHERICHIA-COLI
CHLORAMPHENICOL-ERYTHROMYCIN RESISTANCE MUTATIONS IN A 23S RIBOSOMAL-RNA GENE OF ESCHERICHIA-COLI
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DOI：
10.1128/jb.162.2.551-557.1985
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
MORGAN, EA
中科院分区：
文献类型：
--
作者：
ETTAYEBI, M;PRASAD, SM;MORGAN, EA
在位于多拷贝质粒上的大肠杆菌rRNA操纵子(RRH)中分离到两个氯霉素耐药突变。这两个突变也使人对14个原子的内酯环大环内酯类抗生素产生抗药性，但不会对16个原子的内酯环大环内酯类抗生素或其他核糖体大亚基的抑制剂产生抗药性。用经典的遗传学和重组DNA方法将这两个突变定位在23S RNA基因的154个碱基区。这些区域的DNA测序显示，氯霉素-红霉素抗性是由两个独立分离的突变株23S RNA基因2057位的G-A转换引起的。这些突变影响23S RNA的一个区域，该区域与肽基转移密切相关，并已知与各种肽转移酶抑制剂相互作用。
Two chloramphenicol resistance mutations were isolated in an E. coli rRNA operon (rrh) located on a multicopy plasmid. Both mutations also confer resistance to 14-atom lactone ring macrolide antibiotics, but they do not confer resistance to 16-atom lactone ring macrolide antibiotics or other inhibitors of the large ribosomal subunit. Classic genetic and recombinant DNA methods were used to map the 2 mutations to 154-basepair regions of the 23S RNA genes. DNA sequencing of these regions revealed that chloramphenicol-erythromycin resistance results from a G-to-A transition at position 2057 of the 23S RNA genes of both independently isolated mutants. These mutations affect a region of 23S RNA strongly implicated in peptidyl transfer and known to interact with a variety of peptidyl transferase inhibitors.