Immunohistochemical localization of environmentally induced cytochrome P450IA1 in multiple organs of the marine teleost Stenotomus chrysops (Scup).
Immunohistochemical localization of environmentally induced cytochrome P450IA1 in multiple organs of the marine teleost Stenotomus chrysops (Scup).
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海洋硬骨鱼 Stenotomus chrysops (Scup) 多个器官中环境诱导的细胞色素 P450IA1 的免疫组织化学定位。
DOI：
10.1016/0041-008x(91)90049-k
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发表时间：
1991
影响因子：
3.8
通讯作者：
Hahn,ME
中科院分区：
文献类型：
--
作者：
Stegeman,JJ;Smolowitz,RM;Hahn,ME
细胞色素P450在不同器官和细胞类型中的表达及其功能的差异可以决定这些细胞和器官对外源性物质的反应。本文报道了3，3 ′，4，4 ′-四氯联苯和2，3，7，8-四氯二苯并呋喃处理后，金眼狭口鱼（Stenotomus chrysops（scup））10种器官或系统中细胞色素P450 IA 1（P450 E）的细胞定位。（R. M. Smolowitz，M. E. Hahn和J. J. Stegeman，Drug Metab. 19，113，1991）。在这里，我们描述了P450 IA 1的存在和本地化的scup直接从环境中采样的氯联苯和二苯并呋喃，外新贝德福德港的马萨诸塞州。使用单克隆抗体1-12-3对所选器官（肝脏、肾脏、鳃和心脏）的微粒体进行Western印迹分析，发现每个器官中P450 IA 1的诱导水平。P450 IA 1在这些和其他器官中的定位在通过标准组织学方法制备的切片中确定，并在间接过氧化物酶标记方法中用MAb 1-12-3染色。P450 IA 1在肝脏的多种细胞类型中检测到，包括肝、胰腺和血管组织。肾脏和肠道上皮结构和血管内皮细胞中也显示出显著的P450 IA 1水平。在心脏、鳃、脾、睾丸、卵巢、鼻和脑的内皮细胞中检测到特异性染色，但未检测到其他细胞类型。在心脏中，染色存在于心房和心室的内皮细胞以及冠状血管和大血管的内皮细胞。结果表明，P450 IA蛋白在许多器官的鱼暴露于环境化学品在野生环境中诱导，与细胞定位的模式，如在鱼实验处理已知的诱导剂。P450 IA 1在所有器官的内皮细胞中的强染色支持实验结果，表明内皮细胞是P450 IA 1诱导的主要位点。我们的研究结果进一步表明，免疫组化是一种有用的方法，用于检测P450诱导作为暴露的生物标志物。
Differences in expression of cytochrome P450 forms and their functions in different organs and cell types could determine the response of those cells and organs to xenobiotics. Recently, we described the cellular localization of cytochrome P450IA1 (P450E) induced in 10 organs or organ systems of the fish, Stenotomus chrysops (scup) treated with 3,3′,4,4′-tetrachlorobiphenyl or with 2,3,7,8-tetrachlorodibenzofuran. (R. M. Smolowitz, M. E. Hahn, and J. J. Stegeman, Drug Metab. Dispos.19, 113, 1991). Here we describe the presence and localization of P450IA1 in organs of scup sampled directly from an environment contaminated by chlorinated biphenyls and dibenzofurans, the outer New Bedford Harbor of Massachusetts. Western blot analysis of microsomes from selected organs (liver, kidney, gill, and heart), using monoclonal antibody 1-12-3, revealed induced levels of P450IA1 in each. The localization of P450IA1 in these and other organs was determined in sections prepared by standard histological methods and stained with MAb 1-12-3 in an indirect peroxidase labeling method. P450IA1 was detected in multiple cell types in liver, including hepatic, pancreatic, and vascular tissue. Kidney and gut also showed prominent P450IA1 levels in epithelial structures and in vascular endothelial cells. Specific staining was detected in endothelial cells, but not other cell types, in heart, gill, spleen, testis, ovary, nose, and brain. In heart, the staining was present in the endocardium of atrium and ventricle, and endothelium of the coronary vasculature and great vessels. The results demonstrate that P450IA proteins are induced in many organs of fish exposed to environmental chemicals in the wild, with patterns of cellular localization like those seen in fish experimentally treated with known inducers. The strong staining of P450IA1 in endothelial cells in all organs examined supports experimental results indicating that endothelium is a major site of P450IA1 induction. Our results indicate further that immunohistochemistry is a useful method for detecting P450 induction as a biomarker for exposure.
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脊椎动物（硬骨鱼）心脏内皮细胞色素 P450IA1 的诱导和定位。
影响因子：
3.6
作者：
Stegeman,JJ;Miller,MR;Hinton,DE
通讯作者：
Hinton,DE
大鼠脑中细胞色素 P-450 的免疫组织化学定位
影响因子：
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作者：
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DOI：
--
发表时间：
1989
期刊：
影响因子：
--
作者：
A. Elskus;J. Stegeman
通讯作者：
J. Stegeman
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DOI：
--
发表时间：
1990
期刊：
影响因子：
--
作者：
P. Veld;D. J. Westbrook;B. Woodin;R. Hale;Craig L. Smith;R. Huggett;J. Stegeman
通讯作者：
J. Stegeman
影响因子：
3.9
作者：
KLOEPPERSAMS, PJ;PARK, SS;STEGEMAN, JJ
通讯作者：
STEGEMAN, JJ