Liver Regeneration in Experimental Carbon Tetrachloride Intoxication.∗
Liver Regeneration in Experimental Carbon Tetrachloride Intoxication.∗
复制标题
实验性四氯化碳中毒中的肝脏再生。*
DOI：
10.3181/00379727-102-25357
复制
发表时间：
1959
影响因子：
--
通讯作者：
C. S. Davidson
中科院分区：
文献类型：
--
作者：
C. M. Leevy;R. Hollister;R. Schmid;R. Macdonald;C. S. Davidson
采用氚化胸苷的放射自显影技术研究了单次口服四氯化碳处理的大鼠的肝再生。结果表明，在中毒性肝损伤后，脱氧核糖核酸的合成，表明新细胞的形成，主要发生在肝脏的非坏死区域;再生过程是弥漫性的，涉及腺泡，中间，小叶中心和门静脉周围区域。在肝毒素给药后36至72小时达到最高再生率，此时标记的肝细胞核数量增加超过对照值的250倍; 120小时后，再生过程几乎完成，组织学和放射自显影模式恢复正常。在本实验条件下，无法证明存在用于肝再生的血浆因子。
Summary An autoradiographic technic employing tritiated thymidine was used to study liver regeneration in rats treated with a single oral dose of carbon tetrachloride. Results demonstrate that following toxic liver injury synthesis of desoxyribonucleic acid, indicating new cell formation, occurs chiefly in non-necrotic areas of the liver; the regenerative process is diffuse and involves the acinar, intermediate, centrilobular and periportal regions. The highest rate of regeneration is reached 36 to 72 hours following administration of hepatotoxin, when the number of labeled liver cell nuclei is increased more than 250 fold over control values; after 120 hours, the regenerative process is virtually completed and the histological and autoradiographic pattern returns to normal. Under conditions of this experiment, the presence of a plasma factor for liver regeneration could not be demonstrated.