Acute stress induces down-regulation of large-conductance Ca2+-activated potassium channels in the lateral amygdala
Acute stress induces down-regulation of large-conductance Ca2+-activated potassium channels in the lateral amygdala
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急性应激引起外侧杏仁核大电导 Ca2 激活钾通道的下调
DOI：
10.1113/jphysiol.2011.223784
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发表时间：
2012-02-01
影响因子：
5.5
通讯作者：
Zhao, Ming-gao
中科院分区：
文献类型：
--
作者：
Guo, Yan-yan;Liu, Shui-bing;Zhao, Ming-gao
大电导钙激活钾通道（BKCa）在杏仁核（LA）中高度表达，与应激障碍密切相关，但有关其在应激障碍神经元回路中作用的数据有限。在本研究中，BKCa通道表达的显着减少，在杏仁核的小鼠伴随着焦虑样行为诱导的急性应激。焦虑动物LA神经元的全细胞膜片钳记录显示，由BKCa通道介导的动作电位的快速后超极化（fAHP）显著减少，导致LA神经元的超兴奋性。激活BKCa通道在LA逆转应激诱导的焦虑样行为后的压力。此外，下调BKCa通道显着增加诱发的NMDA受体介导的兴奋性突触后电位在丘脑-LA突触。这些数据表明，第一次，约束应力诱导的焦虑样行为至少可以部分地解释在LA的功能BKCa通道的改变。
Large-conductance Ca2+-activated potassium channels (BKCa) are highly expressed in the lateral amygdala (LA), which is closely involved in assigning stress disorders, but data on their role in the neuronal circuits of stress disorders are limited. In the present study, a significant reduction in BKCa channel expression in the amygdala of mice accompanied anxiety-like behaviour induced by acute stress. Whole-cell patch-clamp recordings from LA neurons of the anxious animals revealed a pronounced reduction in the fast after-hyperpolarization (fAHP) of action potentials mediated by BKCa channels that led to hyperexcitability of the LA neurons. Activation of BKCa channels in the LA reversed stress-induced anxiety-like behaviour after stress. Furthermore, down-regulated BKCa channels notably increased the evoked NMDA receptor-mediated excitatory postsynaptic potentials at the thalamo-LA synapses. These data demonstrate, for the first time, that restraint stress-induced anxiety-like behaviour could at least partly be explained by alterations in the functional BKCa channels in the LA.