Comparative transcriptomic analysis uncovers conserved pathways involved in adventitious root formation in poplar
Comparative transcriptomic analysis uncovers conserved pathways involved in adventitious root formation in poplar
复制标题
比较转录组分析揭示了杨树不定根形成中涉及的保守途径
DOI：
10.1007/s12298-021-01054-7
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发表时间：
2021-08-31
影响因子：
3.5
通讯作者：
Wang, Nian
中科院分区：
文献类型：
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作者：
Luo, Jie;Nvsvrot, Tashbek;Wang, Nian
扦插繁殖是白杨人工林的主要造林方法，但其对不定根的形成有很高的要求。虽然白杨品种被认为是容易形成根，AR形成的种间差异仍然观察。为了更好地了解白杨AR形成过程中保守修饰途径的基因调控网络，采用比较转录组学方法对两种白杨（欧洲杨和欧洲杨）AR形成过程中差异表达的保守共有基因进行了鉴定。simonii）在木本植物培养基（WPM）中培养。共有2146个基因被鉴定为保守基因，在至少一次比较中具有相似的基因表达谱。这些保守基因在不同的激素信号通路中富集，同时也在丝裂原相关蛋白激酶（MAPK）信号通路中富集，表明信号转导在白杨AR调控中协调外部刺激和内源生理状态的重要作用。此外，保守基因的共表达网络分析允许鉴定具有不同生物学功能的几个共表达模块（CM），例如，CM 1在防御反应和激素信号传导中富集，CM 2和CM 3分别在防御反应相关途径和细胞周期中过量表达。这些结果表明，在转录组水平上，白杨AR形成过程进行了微调，通过整合AR形成所必需的多种生物过程。我们的研究结果表明，保守的机制，在白杨AR的形成和产生的信息基因共表达网络，描述了在这些物种的AR形成的基础。
Cutting propagation is widely used in establishing poplar plantations, and this approach requires efficient adventitious root (AR) forming capacities. Although poplar species are considered to form roots easily, interspecific variations in AR formation are still observed. To better understand the gene regulatory network underlying the conserved modified pathways that are essential for AR formation in poplar species, comparative transcriptomic approaches were applied to identify the conserved common genes that were differentially expressed during the AR formation processes in two poplar species (Populus×euramericanaandP. simonii) in woody plant medium (WPM). A total of 2146 genes were identified as conserved genes that shared similar gene expression profiles in at least one comparison. These conserved genes were enriched in diverse hormone signaling pathways, as well as the mitogen-associated protein kinase (MAPK) signaling pathway, suggesting an important role for signaling transduction in coordinating external stimuli and endogenous physiological status during AR regulation in poplar. Furthermore, the co-expression network analysis of conserved genes allowed identification of several co-expressed modules (CM) that are co-expressed with distinct biological functions, for instance, CM1 was enriched in defense response and hormone signaling, CM2 and CM3 were overrepresented in defense response-related pathways and for cell cycle, respectively. These results suggest that the AR formation processes in poplar were finely tuned at the transcriptomic level by integrating multiple biological processes essential for AR formation. Our results suggest conserved machinery for AR formation in poplar and generated informative gene co-expression networks that describe the basis of AR formation in these species.