Apheresis-induced platelet activation:: comparison of three types of cell separators
Apheresis-induced platelet activation:: comparison of three types of cell separators
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DOI：
10.1046/j.1537-2995.2000.40020182.x
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发表时间：
2000-02-01
期刊：
影响因子：
2.9
通讯作者：
Kjeldsen-Kragh, J
中科院分区：
文献类型：
--
作者：
Hagberg, IA;Akkok, ÇA;Kjeldsen-Kragh, J
背景：血小板采集技术在不同的细胞分离器中存在差异。研究设计和方法：使用三种细胞分离器的四组（n = 10）单针单采程序进行比较：1）光谱LRS，90分钟收获时间; 2）MCS+，90分钟收获; 3）Amicus，90分钟收获;（4）Amicus，45分钟。在单采停止后的前4小时内，每隔一段时间从供体采集全血样本以及最终组分样本。采用流式细胞术检测血小板活化状态及激动剂刺激后血小板活化能力。结果：供者单采前后均未检测到活化血小板。Amicus（90分钟）组分中的血小板比其他组分组中的血小板活化程度显著更高：即血小板聚集体大小增加、微粒分数增加、脱粒增加、纤维蛋白原受体活化增加和血管性血友病因子受体表达降低。此外，这些血小板对激动剂刺激的反应降低了所有激活variable.CONCLUSIONS：90分钟的处理时间后，与Amicus细胞分离器获得的血小板比与光谱和MCS+收获的血小板显着更活化。
BACKGROUND: Platelet-harvesting technology differs in various cell separators. Alteration in shear stress and biocompatibility of surfaces may give variable platelet activation and thereby affect the quality of the component.STUDY DESIGN AND METHODS: Four groups (n = 10) of single-needle apheresis procedures using three cell separators, were compared: 1) Spectra LRS, 90-minute harvesting time; 2) MCS+, 90-minute harvest; 3) Amicus, 90-minute; and 4) Amicus, 45-minute. Whole-blood samples were collected from the donors as were samples from the final components at intervals during the first 4 hours after cessation of the apheresis. Platelet activation status and platelet activation capacity after agonist stimulation were assessed by flow cytometry.RESULTS: No activated platelets were found in preapheresis and postapheresis samples from the donors. The platelets in the components from the Amicus (90-min) were significantly more activated than those in the other groups of components: that is, there was increased size of platelet aggregates, increased fraction of microparticles, increased degranulation, increased fibrinogen receptor activation, and decreased von Willebrand factor receptor expression. Moreover, the response of these platelets to agonist stimulation was reduced for all activation variables.CONCLUSIONS: After 90 minutes' processing time, platelets obtained with the Amicus cell separator were significantly more activated than platelets harvested with the Spectra and the MCS+.