Design of Regulated Ion Channels Using Measurements of Cis-Trans Isomerization in Single Molecules
Design of Regulated Ion Channels Using Measurements of Cis-Trans Isomerization in Single Molecules
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DOI：
10.1021/ja00121a002
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发表时间：
1995-04
影响因子：
15
通讯作者：
G. Woolley;A. Jaikaran;Zhi-hua Zhang;Shu Peng
中科院分区：
文献类型：
--
作者：
G. Woolley;A. Jaikaran;Zhi-hua Zhang;Shu Peng
人工通道设计的一个关键组成部分是监管要素的设计。我们已经合成了一种肽离子通道的衍生物，gramicidin，其中一个氨基通过含有氨基甲酸酯基团的连接剂连接到通道的口。氨基甲酸酯的热顺反异构化可以在单分子水平上检测到，它可以调节Cs+离子通过通道的通量。该系统的可预测性表明，设计更复杂的渠道监管类型将是可能的。
A key component in the design of artificialion channels is the design of regulatory elements. We have synthesized a derivative of the peptide ion channel, gramicidin, in which an amino group is connected to the mouth of the channelby a linker containing a carbamate group. Thermal cis-trans isomerization of the carbamate, which can be detected at the single-molecule level, is found to regulate the flux of Cs+ ions through the channel. The predictability of the system suggests that design of more sophisticated types of channel regulation will be possible.