Compositional Changes in Cell Wall Polysaccharides from Japanese Plum (Prunus salicina Lindl.) during Growth and On-Tree Ripening
Compositional Changes in Cell Wall Polysaccharides from Japanese Plum (Prunus salicina Lindl.) during Growth and On-Tree Ripening
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DOI：
10.1021/jf9039099
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发表时间：
2010-02-24
影响因子：
6.1
通讯作者：
Sozzi, Gabriel O.
中科院分区：
文献类型：
--
作者：
Ponce, Nora M. A.;Ziegler, Victor H.;Sozzi, Gabriel O.
采自6个不同发育阶段的跃变型日本李，不设中间贮藏期，分析细胞壁组成的变化。在生长过程中，阿拉伯糖是细胞壁中的主要中性单糖成分，果胶组分中的中性糖含量最高。紧密结合的果胶的阿拉伯糖损失被发现是细胞壁生化修饰序列中相对较早的特征，因此表明在日本李树上成熟过程中软化相关的作用。基质多糖的解聚开始于细胞扩张期的末期，并在整个成熟过程中不断增加。果胶的增溶作用首先在早熟时被检测到。硬度下降与果胶成熟早期多糖醛酸解聚无关，但最后软化阶段与细胞壁多糖解聚以及松散结合果胶中阿拉伯糖/半乳糖比值的降低有关。这是描述日本李细胞壁多糖变化的时间序列的第一项工作。
Climacteric Japanese plums were harvested at six developmental stages with no intermediate storage period, and cell wall compositional changes were analyzed. Arabinose proved to be the principal neutral monosaccharide constituent in cell walls during growth and the most dynamic neutral sugar in pectic fractions. Arabinose loss from tightly bound pectins was found to be a relatively early feature in the sequence of cell wall biochemical modifications, thus suggesting a softening-related role during Japanese plum on-tree ripening. Depolymerization of matrix glycans started at the end of the cell expansion phase and increased throughout ripening. Pectin solubilization was first detected during early ripening. Firmness loss did not correlate with polyuronide depolymerization early in ripening, but the last softening phase was associated with a strong depolymerization of cell wall polyuronides as well as a decrease in the arabinose/galactose ratio in loosely bound pectins. This is the first work that characterizes the temporal sequence of cell wall polysaccharide changes in Japanese plum.