The DiversiLab system versus pulsed-field gel electrophoresis: Characterisation of extended spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae
The DiversiLab system versus pulsed-field gel electrophoresis: Characterisation of extended spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae
复制标题
DOI：
10.1016/j.mimet.2010.09.004
复制
发表时间：
2010-11-01
影响因子：
2.2
通讯作者：
Giske, Christian G.
中科院分区：
文献类型：
--
作者：
Brolund, Alma;Haeggman, Sara;Giske, Christian G.
迫切需要快速、可靠的流行病学分型方法来鉴定产超广谱β-内酰胺酶(ESBL)肠杆菌科细菌的暴发和流行菌株。DiversiLab系统(DL)就是为这些目的而提出的。我们对2007年全国分离的产超广谱β-内酰胺酶的大肠埃希菌(n=258，其中226株可用脉冲场凝胶电泳法分型)和肺炎克雷伯菌(n=48)进行了DL和脉冲场凝胶电泳法的比较。对于大肠杆菌，Wallace系数表明，同一DL型的两个分离物具有相同的PFGE型的概率仅为19.8%，而同一PFGE型的两个分离物具有相同的DL型的概率为90.4%。对于肺炎克雷伯菌，Wallace系数显示同一DL型的两个分离物具有相同的PFGE类型的概率为100%，同一PFGE类型的两个分离物具有相同的DL型的概率为79%，这表明对于该肺炎克雷伯菌的DL型收集稍微更具区分性。48株分离物中只有4株与两种方法的结果不一致。在大肠杆菌中，42%的分离物为131型，这些分离物与DL的同源性为95%，与PFGE的同源性为60%。总而言之，对于E.ColiDl，在鉴定与PFGE相关的菌株方面表现良好，但高估了所研究样本中的遗传相关性。这表明DL可作为排除无关菌株的一种初步筛选方法。被证明有亲缘关系的分离株必须用更具歧视性的方法确认。对于肺炎克雷伯菌，与PFGE相比，DL能很好地区分，但高估了分离株的多样性，假设有可能遗漏可能的遗传联系的风险。(C)2010爱思唯尔B.V.保留所有权利。
Fast and reliable epidemiological typing methods for identifying outbreaks and epidemic strains of extended spectrum p-lactamase (ESBL) producing Enterobacteriaceae are urgently needed. The DiversiLab system (DL) has been proposed for these purposes. We compared DL to pulsed-field gel electrophoresis (PFGE) on a national collection of ESBL-producing Escherichia coli (n = 258; of which 226 isolates were typeable with PFGE) and Klebsiella pneumoniae (n = 48) isolated in 2007. For E. coli the Wallace coefficients showed that the probability of two isolates of the same DL type having the same PFGE type was only 19.8% and the probability of two isolates of the same PFGE type having the same DL type was 90.4%. For K. pneumoniae the Wallace coefficients showed that the probability of two isolates of the same DL type having the same PFGE type was 100% and the probability of two isolates of the same PFGE type having the same DL type was 79%, indicating that for this K. pneumoniae strain collection DL was slightly more discriminatory. Only four of 48 isolates had discordant results with the two methods. In E. coli 42% of the isolates were sequence type 131 and these isolates were related at >95% similarity with DL and at >= 60% similarity with PFGE. In summary, for E. coli DL performed well in identifying isolates related by PFGE, but overestimated the genetic relatedness in the studied collection. This indicates that DL could be a primary screening method for excluding unrelated isolates. Isolates shown to be related must be confirmed with a more discriminatory method. For K. pneumoniae, DL discriminated well but overestimated the diversity of the isolates compared to PFGE, assuming a risk of missing possible genetic relatedness. (C) 2010 Elsevier B.V. All rights reserved.