Disruption of circumferential actin filament causes disappearance of occludin from the cell borders of rat hepatocytes in primary culture without distinct changes of tight junction strands
Disruption of circumferential actin filament causes disappearance of occludin from the cell borders of rat hepatocytes in primary culture without distinct changes of tight junction strands
复制标题
DOI：
10.1247/csf.24.11
复制
发表时间：
1999-02-01
影响因子：
1.5
通讯作者：
Mochizuki, Y
中科院分区：
文献类型：
--
作者：
Kojima, T;Sawada, N;Mochizuki, Y
我们使用肌动蛋白解聚剂B研究了原代培养大鼠肝细胞中肌动蛋白丝组织与闭合蛋白和紧密连接链的关系。在对照培养物中，发达的圆周肌动蛋白丝和occludin免疫反应性观察到的最subapical质膜的细胞，和紧密连接链形成发达的网络在冷冻断裂复制品。在3 μ M mycalcantine B处理6 h的肝细胞中，细胞边缘的周向肌动蛋白丝和occludin免疫反应性消失，然而，冷冻断裂复制品中的紧密连接链没有明显异常。在含0.05mM Ca ~（2+）的培养基中培养6小时的细胞也得到类似的结果。闭合蛋白与肌动蛋白的紧密结合以及几乎不含闭合蛋白和肌动蛋白的完整紧密连接链的存在表明闭合蛋白在肌动蛋白细胞骨架和紧密连接之间的连接中具有生理作用，而不是形成紧密连接链的其他蛋白质。
We investigated the relationship of actin filament organization to occludin and tight junction strands in primary cultured rat hepatocytes using an actin depolymerizing agent, mycalolide B. In control cultures, well-developed circumferential actin filaments and occludin immunoreactivity were observed on the most subapical plasma membrane of the cells, and tight junction strands formed well-developed networks in freeze-fracture replicas. In hepatocytes treated with 3 mu M mycalolide B for 6 h, circumferential actin filaments and occludin immunoreactivity disappeared from the cell borders, However, there were no marked abnormalities of tight junction strands in freeze fracture replicas. Similar results were obtained from cells cultured in medium with 0.05 mM Ca2+ for 6 h. The close association of occludin with actin and the existence of intact tight junction strands that are virtually free of both occludin and actin suggest a physiological role of occludin, but not the other proteins forming the tight junction strands, in the linkage between actin cytoskeleton and tight junction.