Relationship between Sperm Hyaluronan-binding Assay (HBA) Scores on Embryo Development, Fertilisation, and Pregnancy Rate in Patients Undergoing Intra-cytoplasmic Sperm Injection (ICSI)
Relationship between Sperm Hyaluronan-binding Assay (HBA) Scores on Embryo Development, Fertilisation, and Pregnancy Rate in Patients Undergoing Intra-cytoplasmic Sperm Injection (ICSI)
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DOI：
10.1177/201010581302200207
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发表时间：
2013-06-01
影响因子：
0.5
通讯作者：
Yu, Su Ling
中科院分区：
文献类型：
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作者：
Fen, Carine To Chiou;Lee, Shaw Ni;Yu, Su Ling
精子透明质酸结合测定（HBA）的新发展目前已在世界各地的一些实验室常规使用，以预测精子的成熟度和功能。因此，本研究的目的是评估低HBA值是否会影响胚胎发育、质量、受精和妊娠率。方法：回顾性比较192例接受胞浆内精子注射（ICSI）的女性患者的胚胎发育、受精率和妊娠率及其丈夫的HBA评分。患者的丈夫被要求在刺激周期开始前进行HBA测试，以确定他们的精液是否适合进行ICSI或体外受精（IVF）。p值< 0.05被认为是显著的。结果：患者分为A组（HBA卡15% < 35%）、C组（HBA卡>= 35% < 60%）、D组（HBA卡>= 60%）4组。A、B、C、D组受精率分别为67.9%、73.1%、72.5%、77.1%。D组受精率显著高于其余各组（p = 0.016）。四组中D组的妊娠率也显著高于B组（p = 0.041），而B组和C组的妊娠率相似（分别为42.4%和41.1%）。第3天，与其他组相比，D组的卵裂率（达到6个及以上细胞的能力）最高（p = 0.002）。结论：HBA评分越高，受精率、妊娠率、乳沟率越好。这表明HBA确实有能力选择染色体发育正常且具有卵母细胞结合能力的成熟精子。
Introduction: The novel development of the sperm hyaluronan-binding assay (HBA) has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. Hence, the purpose of this study is to evaluate if embryo development, quality, fertilisation and pregnancy rates are affected by low HBA values.Methods: A total of192 female patients who underwent intra-cytoplasmic sperm injection (ICSI) were compared retrospectively in terms of embryo development, fertilisation, and pregnancy rates with their husbands' HBA score. Patients' husbands were required to undergo a HBA test before the start of their stimulation cycle to determine if their semen was suitable to undergo ICSI or in-vitro fertilisation (IVF). P-value < 0.05 was considered significant.Results: Patients were divided into four groups, group A (HBA 15% < 35%), group C (HBA >= 35% < 60%), and group D (HBA >= 60%). The fertilisation rate for groups A, B, C, and D were 67.9%, 73.1%, 72.5%, and 77.1% respectively. Group D had a fertilisation rate significantly higher than the rest of the groups (p = 0.016). The pregnancy rate for group D was also significantly higher amongst the four groups (p = 0.041), whereas the pregnancy rate for groups B and C was similar (42.4% versus 41.1% respectively). Day three cleavage rate (the ability to reach six cells and beyond) was highest for group D compared to the other groups (p = 0.002).Conclusion: The higher the HBA score, the better the fertilisation, pregnancy, and cleavage rates. This shows that HBA does have the ability to select mature sperms with normal chromosome development and oocyte-binding capability.