The importance of hyaluronic acid in vocal fold biomechanics
The importance of hyaluronic acid in vocal fold biomechanics
复制标题
DOI：
10.1067/mhn.2001.115906
复制
发表时间：
2001-06-01
影响因子：
3.4
通讯作者：
Titze, IR
中科院分区：
文献类型：
--
作者：
Chan, RW;Gray, SD;Titze, IR
目的：本研究探讨了透明质酸（HA）对人声带覆盖物生物力学性能的影响（固有层的表层）。研究设计：从5具成年男性尸体新鲜切除声带组织，并用牛睾丸透明质酸酶处理，以选择性地从固有层细胞外基质（ECM）中去除HA，线性粘弹性剪切特性在酶处理之前和之后，将组织样品的弹性剪切模量和动态粘度定量为频率的函数（0.01至15 Hz）。在从声带ECM去除HA时，声带覆盖物的弹性剪切模量（G '）或刚度平均降低约35%，而在更高频率（> 1Hz）时，动态粘度（eta '）增加了70%.结论：HA对声带的生物力学性能有重要的影响.作为声带细胞外基质中的高度水合糖胺聚糖，它可能有助于维持最佳的组织粘度，这可能有助于发声，以及最佳的组织硬度，这可能对声音基本频率控制很重要。意义：HA已被提议作为一种潜在的生物植入物用于手术修复声带细胞外基质缺陷（例如，声带疤痕和声沟）。我们的研究结果表明，从生物力学的角度来看，这种临床应用可能是语音产生的最佳选择。
OBJECTIVE: This study examined the influence of hyaluronic acid (HA) on the biomechanical properties of the human vocal fold cover (the superficial layer of the lamina propria).STUDY DESIGN: Vocal fold tissues were freshly excised from 5 adult male cadavers and were treated with bovine testicular hyaluronidase to selectively remove HA from the lamina propria extracellular matrix (ECM), Linear viscoelastic shear properties (elastic shear modulus and dynamic viscosity) of the tissue samples before and after enzymatic treatment were quantified as a function of frequency (0.01 to 15 Hz) by a parallel-plate rotational rheometer at 37 degreesC.RESULTS: On removing HA from the vocal fold ECM, the elastic shear modulus (G ') or stiffness of the vocal fold cover decreased by an average of around 35%, while the dynamic viscosity (eta ') increased by 70% at higher frequencies (> 1 Hz).CONCLUSION: The results suggested that HA plays an important role in determining the biomechanical properties of the vocal fold cover. As a highly hydrated glycosaminoglycan in the vocal fold ECM, it likely contributes to the maintenance of an optimal tissue viscosity that may facilitate phonation, and an optimal tissue stiffness that may be important for vocal fundamental frequency control.SIGNIFICANCE: HA has been proposed as a potential bioimplant for the surgical repair of vocal fold ECM defects (eg, vocal fold scarring and sulcus vocalis). Our results suggested that such clinical use may be potentially optimal for voice production from a biomechanical perspective.