Influence of hydrodynamic conditions on quantitative cellular assays in microfluidic systems
Influence of hydrodynamic conditions on quantitative cellular assays in microfluidic systems
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DOI：
10.1021/ac071146k
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发表时间：
2007-09-15
影响因子：
7.4
通讯作者：
Cooper, Jonathan M.
中科院分区：
文献类型：
--
作者：
Yin, Huabing;Zhang, Xunli;Cooper, Jonathan M.
本研究证明了微流体系统中流体动力环境在使用活细胞进行定量细胞分析中的重要性。以中国仓鼠卵巢（CHO）细胞内Ca2+定量分析为模型系统，对微流体中常用的流动条件进行了评价。在一定的剪切应力阈值以上，观察到流体动力诱导的细胞内Ca2+通量，其模拟化学刺激诱导的反应，如激动剂尿苷5'-三磷酸三盐（UTP）。鉴于微流控装置在生物物理应用和药物筛选的高通量细胞分析中的应用越来越多，朱鹭效应具有重要意义。
This study demonstrates the importance of the hydrodynamic environment in microfluidic systems in quantitative cellular assays using live cells. Commonly applied flow conditions used in microfluidics were evaluated using the quantitative intracellular Ca2+ analysis of Chinese hamster ovary (CHO) cells as a model system. Above certain thresholds of shear stress, hydrodynamically induced intracellular Ca2+ fluxes were observed which mimic the responses induced by chemical stimuli, such as the agonist uridine 5'-triphosphate tris salt (UTP). Ibis effect is of significance given the increasing application of microfluidic devices in high-throughput cellular analysis for biophysical applications and pharmacological screening.