Antibody response against a Leishmania donovani amastigote-stage-specific protein in patients with visceral leishmaniasis
Antibody response against a Leishmania donovani amastigote-stage-specific protein in patients with visceral leishmaniasis
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DOI：
10.1128/cdli.4.5.530-535.1997
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发表时间：
1997-09-01
期刊：
影响因子：
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通讯作者：
Matlashewski, G
中科院分区：
文献类型：
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作者：
Ghedin, E;Zhang, WW;Matlashewski, G
研究了杜氏利什曼原虫无鞭毛体特异性蛋白(A2)的抗体反应。对杜氏锥虫病和各种形式利什曼病患者的血清进行抗A2抗体筛选，发现仅感染华支睾吸虫和墨西哥利什曼原虫的患者血清特异性识别A2重组蛋白，这些结果与核型分析结果一致，表明该A2基因在杜氏锥虫和墨西哥利什曼原虫株中是保守的。通过筛选从杜诺瓦尼乳杆菌流行的苏丹和印度地区的患者获得的一系列血清，进一步探索该抗原在诊断中的潜力，所有样本的抗A2抗体的流行率通过Western blotting确定。还对部分样品进行了酶联免疫吸附试验(ELISA)和免疫沉淀试验。来自苏丹和印度的活动性内脏利什曼病(黑热病)患者血清中，抗A2抗体阳性率分别为82%和60%，而免疫沉淀法检出抗体的阳性率为92%，提示A2蛋白可作为内脏利什曼病的诊断抗原。
The antibody response against an amastigote-specific protein (A2) from Leishmania donovani was investigated. Sera from patients with trypanosomiasis and various forms of leishmaniasis were screened for anti-A2 antibodies, Sera from patients infected only with L. donovani or Leishmania mexicana specifically recognized the A2 recombinant protein, These results were consistent with karyotype analyses which revealed that the A2 gene is conserved in L. donovani and L. mexicana strains. The potential of this antigen in diagnosis was further explored by screening a series of sera obtained from patients in regions of the Sudan and India where L. donovani is endemic, The prevalence of anti-A2 antibodies was determined by Western blotting for all samples. Enzyme-linked immunosorbent assay (ELISA) and an immunoprecipitation assay were also performed on some of the samples. Anti-A2 antibodies were detected bg ELISA in 82 and 60% of the samples from individuals with active visceral leishmaniasis (kala-azar) from the Sudan and India, respectively, while the immunoprecipitation assay defected the antibodies in 92% of the samples from India, These data suggest that the A2 protein mag be a useful diagnostic antigen for visceral leishmaniasis.