Translesion synthesis: Y-farnily polymerases and the polymerase switch
Translesion synthesis: Y-farnily polymerases and the polymerase switch
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DOI：
10.1016/j.dnarep.2007.02.003
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发表时间：
2007-07-01
期刊：
影响因子：
3.8
通讯作者：
Green, Catherine M.
中科院分区：
文献类型：
--
作者：
Lehmann, Alan R.;Niimi, Atsuko;Green, Catherine M.
复制性DNA聚合酶在DNA损伤处被阻断。DNA损伤后的合成需要用一组专门的跨损伤合成（TLS）聚合酶之一取代复制聚合酶，其中大多数属于Y家族。每一种都对不同类型的损伤具有不同的底物特异性。在真核生物中，PCNA的单泛素化在停滞分叉处从复制型聚合酶到TLS聚合酶的转换中起着至关重要的作用。所有的Y家族聚合酶都具有泛素结合位点，其增加了它们在停滞叉位点处与泛素化PCNA的结合亲和力。(C)2007年由Elsevier B.V.出版。
Replicative DNA polymerases are blocked at DNA lesions. Synthesis past DNA damage requires the replacement of the replicative polymerase by one of a group of specialised translesion synthesis (TLS) polymerases, most of which belong to the Y-family. Each of these has different substrate specificities for different types of damage. In eukaryotes mono-ubiquitination of PCNA plays a crucial role in the switch from replicative to TLS polymerases at stalled forks. All the Y-family polymerases have ubiquitin binding sites that increase their binding affinity for ubiquitinated PCNA at the sites of stalled forks. (C) 2007 Published by Elsevier B.V.