Enhanced enzyme saccharification of Sorghum bicolor straw using dilute alkali pretreatment
Enhanced enzyme saccharification of Sorghum bicolor straw using dilute alkali pretreatment
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DOI：
10.1016/j.biortech.2010.03.116
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发表时间：
2010-09-01
影响因子：
11.4
通讯作者：
Vancov, T.
中科院分区：
文献类型：
--
作者：
McIntosh, S.;Vancov, T.
研究了不同预处理参数（温度、时间和碱度）对高粱秸秆酶解的影响。预处理后，固体和木质素含量被发现是成反比的严重程度的治疗。较高的温度和碱强度是最大限度地从酶糖化中回收糖的关键。当高粱秸秆在2%NaOH中在121 ° C下预处理60分钟时，总糖释放达到峰值;与在不存在碱的情况下在60 ° C下预处理的样品相比，代表5.6倍的高产率。类似地，在60 ° C下用2%NaOH处理90分钟的样品的总糖增加了4.3倍，证实了碱包含的重要性。添加β-葡萄糖苷酶和木聚糖酶的糖化混合物提高反应速率和最终糖产量，同时减少纤维素酶的用量4倍。预处理后的固形物的澄清效率分别接近90%和95%（w/w），仅用2.5和5.0FPU纤维素酶/g。皇冠版权所有（C）2010由爱思唯尔有限公司出版。保留所有权利。
The impacts of varying pretreatment parameters (temperature, time, and alkalinity) on enzymatic hydrolysis of sorghum straw were investigated. Following pretreatment, both solids and lignin content was found to be inversely proportional to the severity of the treatments. Higher temperatures and alkali strength were quintessential for maximising sugar recoveries from enzyme saccharifications. Total sugar release peaked when sorghum straw was pretreated in 2% NaOH at 121 degrees C for 60 min; representing a 5.6-fold higher yield compared to samples pretreated at 60 degrees C in the absence of alkali. Similarly, 4.3-fold increases in total sugars from samples treated with 2% NaOH at 60 degrees C for 90 min, confirmed the importance of alkali inclusion. Addition of p-glucosidase and xylanase to saccharification mixtures enhanced reaction rates and final sugar yields, whilst reducing cellulase dosage 4-fold. Saccharification efficiency of pretreated solids approached 90% and 95% (w/w) with as little as 2.5 and 5.0 FPU cellulase/g, respectively. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.