Isolation of Mycoplasmas from tissue cell lines and transplantable tumor cells.
Isolation of Mycoplasmas from tissue cell lines and transplantable tumor cells.
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从组织细胞系和可移植肿瘤细胞中分离支原体。
DOI：
10.1111/j.1348-0421.1967.tb00347.x
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发表时间：
1967
期刊：
影响因子：
--
通讯作者：
K. Koshimizu
中科院分区：
文献类型：
--
作者：
M. Ogata;K. Koshimizu
对日本实验室保存的细胞系和可移植肿瘤系中的支原体污染进行了调查。共检测了60株细胞系，其中52株（86.7%）被支原体污染。这些支原体分离自人源细胞系10/13（77%）、猴源细胞系3/3（100%）、大鼠源细胞系14/19（74%）和小鼠源细胞系25/25（100%）。99份肿瘤标本中均未发现支原体。从细胞系中初步分离出支原体菌落后，可以分化为三种形态类型。然而，所有的殖民地类型表现出相似的生物学特性。除一株支原体外，所有分离的支原体均可通过生长抑制试验和荧光抗体技术鉴定为人型支原体1。
A survey was made of mycoplasma contamination in cell lines and transplantable tumor lines maintained in laboratories in Japan. A total of 60 cell lines were tested and 52 (86.7%) of which were contaminated by mycoplasmas. These mycoplasmas were isolated from the cell lines of human origin 10/13 (77%), from monkeys 3/3 (100%), from rats 14/19 (74%) and from mice 25/25 (100%). Mycoplasmas were not found in any of the 99 samples of tumor materials. Mycoplasma colonies, upon primary isolation from cell lines, could be differentiated into three morphological types. However, all colonial types showed similar biological properties. All but one of the isolated mycoplasmas could be identified as Mycoplasma hominis type 1 by both growth inhibition tests and by fluorescent antibody technique.